Abstract
We developed improved liposomes that produce efficacy for the treatment of cancer, cardiovascular diseases, and HIV-1-related diseases in small and large animal models. Because our processes are reproducible, we have standard operating procedures (SOPs) for the cGMP manufacture of these reagents that have been approved by the Food and Drug Administration for use in phase I/II clinical trials.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Ramesh, R., Saeki, T., Templeton, N. S., Ji, L., Stephens, L. C., Ito, I., Wilson, D. R., Wu, Z., Branch, C. D., Minna, J. D., and Roth, J. A. (2001) Successful treatment of primary and disseminated human lung cancers by systemic delivery of tumor suppressor genes using an improved liposome vector. Mol Ther 3, 337–350.
Shi, H. Y., Liang, R., Templeton, N. S., and Zhang, M. (2002) Inhibition of breast tumor progression by systemic delivery of the maspin gene in a syngeneic tumor model. Mol Ther 5, 755–761.
Tirone, T. A., Fagan, S. P., Templeton, N. S., Wang, X. P., and Brunicardi, F. C. (2001) Insulinoma induced hypoglycemic death in mice is prevented with beta cell specific gene therapy. Ann Surg 233, 603–611.
Yotnda, P., Chen, D.-H., Chiu, W., Piedra, P. A., Davis, A., Templeton, N. S., and Brenner, M. K. (2002) Bilamellar cationic liposomes protect adenovectors from preexisting humoral immune responses. Mol Ther 5, 233–241.
Templeton, N. S., Lasic, D. D., Frederik, P. M., Strey, H. H., Roberts, D. D., and Pavlakis, G. N. (1997) Improved DNA: liposome complexes for increased systemic delivery and gene expression. Nature Biotech 15, 647–652.
Lu, H., Zhang, Y., Roberts, D. D., Osborne, C. K., and Templeton, N. S. (2002) Enhanced gene expression in breast cancer cells in vitro and tumors in vivo. Mol Ther 6, 783–792.
Templeton, N. S. (2002) Liposomal delivery of nucleic acids in vivo. DNA Cell Biol 21, 857–867.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Templeton, N.S. (2010). Liposomes for Gene Transfer in Cancer Therapy. In: Yotnda, P. (eds) Immunotherapy of Cancer. Methods in Molecular Biology, vol 651. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-786-0_15
Download citation
DOI: https://doi.org/10.1007/978-1-60761-786-0_15
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-60761-785-3
Online ISBN: 978-1-60761-786-0
eBook Packages: Springer Protocols