Abstract
Zebrafish behavioral phenotypes are often evaluated in response to pharmacological modulation by various psychotropic drugs. An important step in this process is the method of drug administration. While the most popular drug administration technique in zebrafish research is by immersion, systemic intraperitoneal injection is another effective alternative. This method is useful for drugs that are difficult to dissolve in water, or which require a better control over the amount of drug delivered to an individual animal. Here we outline a simple protocol for the intraperitoneal injection of drugs in adult zebrafish.
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References
Wong, K. et al. Analyzing habituation responses to novelty in zebrafish (Danio rerio). Behav. Brain Res. 208(2), 450–457 (2010).
Egan, R.J. et al. Understanding behavioral and physiological phenotypes of stress and anxiety in zebrafish. Behav. Brain Res. 205(1), 38–44 (2009).
Bencan, Z., Sledge, D., & Levin, E.D. Buspirone, chlordiazepoxide and diazepam effects in a zebrafish model of anxiety. Pharmacol. Biochem. Behav. 94(1), 75–80 (2009).
Levin, E.D., Bencan, Z., & Cerutti, D.T. Anxiolytic effects of nicotine in zebrafish. Physiol. Behav. 90(1), 54–58(2007).
Cachat, J. et al. Modeling withdrawal syndrome in zebrafish. Behav. Brain Res. 205, 38–44 (2009).
Grossman, L. et al. Characterization of behavioral and endocrine effects of LSD on zebrafish. Behav. Brain Res. 214(2), 277–284 (2010).
Wong, K. et al. Modeling seizure-related behavioral and endocrine phenotypes in adult zebrafish. Brain Res. 1348, 209–215 (2010).
Klaassen, C.D. (2001) Casarett & Doull’s Toxicology – The Basic Science of Poisons (6th Edition). McGraw-Hill, New York.
March, T.H. et al. Inhalation administration of all-trans-retinoic acid for treatment of elastase-induced pulmonary emphysema in Fischer 344 rats. Exp. Lung Res. 30(5), 383–404 (2004).
Muneoka, K. et al. Prenatal nicotine exposure affects the development of the central serotonergic system as well as the dopaminergic system in rat offspring: involvement of route of drug administrations. Brain Res. Dev. Brain Res. 102(1), 117–126 (1997).
Gerlai, R., Lee, V., & Blaser, R. Effects of acute and chronic ethanol exposure on the behavior of adult zebrafish (Danio rerio). Pharmacol. Biochem. Behav. 85(4), 752–761 (2006).
Sackerman, J. et al. Zebrafish behavior in novel environments: effects of acute exposure to anxiolytic compounds and choice of Danio rerio line. Inter. J. Compar. Psychol. 23(1), 43–61 (2010).
Kato, Y., Onishi, H., & Machida, Y. N-succinyl-chitosan as a drug carrier: water-insoluble and water-soluble conjugates. Biomaterials 25(5), 907–915 (2004).
Tiersch, T.R. & Griffith, J.S. Apomorphine-induced vomiting in rainbow trout (Salmo gairdneri). Comp. Biochem. Physiol. A Comp. Physiol. 91(4), 721–725 (1988).
Samuelsen, O.B. & Ervik, A. Single dose pharrnacokinetic study of flumequine after intravenous, intraperitoneal and oral administration to Atlantic halibut (Hippoglossus hippoglossus) held in seawater at 9°C. Aquaculture 158, 215–227 (1997).
Tagliari, K.C. et al. Oxidative stress damage in the liver of fish and rats receiving an intraperitoneal injection of hexavalent chromium as evaluated by chemiluminescence. Env. Toxic. Pharm. 17(3), 149–157 (2004).
Zhang, X. et al. Hematological and plasma biochemical responses of crucian carp (Carassius auratus) to intraperitoneal injection of extracted microcystins with the possible mechanisms of anemia. Toxicon 49(8), 1150–1157 (2007).
Pollard, H.B., et al. A parkinsonian syndrome induced in the goldfish by the neurotoxin MPTP. FASEB J. 6(12), 3108–3116 (1992).
Mennigen, J.A. et al. Effects of fluoxetine on the reproductive axis of female goldfish (Carassius auratus). Physiol. Genomics 35(3), 273–282 (2008).
Lushchak, V.I. et al. Diethyldithiocarbamate injection induces transient oxidative stress in goldfish tissues. Chem. Biol. Interact. 170(1), 1–8 (2007).
Hibbert, B. et al. Catecholamine depletion modulates serum LH levels, GAD67 mRNA, and GABA synthesis in the goldfish. Gen. Comp. Endocrinol. 140(3), 176–183 (2005).
Garina, D.V., Kuz’mina, V.V., & Gerasimov, Y.V. The effect of epinephrine on feeding and motion patterns in goldfish Carassius auratus (L.). Comp. Biochem. Physiol. A Mol. Integr. Physiol. 148(3), 544–549 (2007).
Al-Hussinee, L. et al. Viral haemorrhagic septicaemia virus IVb experimental infection of rainbow trout, Oncorhynchus mykiss (Walbaum), and fathead minnow, Pimphales promelas (Rafinesque). J. Fish Dis. 33(4), 347–360 (2010).
Grove, D.J. The effects of adrenergic drugs on melanophores of the minnow, Phoxinus phoxinus (L.). Comp. Biochem. Physiol. 28(1), 37–54 (1969).
Winter, M.J., Ellis, L.C., & Hutchinson, T.H. Formation of micronuclei in erythrocytes of the fathead minnow (Pimephales promelas) after acute treatment with mitomycin C or cyclophosphamide. Mutat. Res. 629(2), 89–99 (2007).
Chettri, J.K. et al. Protective immunization against Tetrahymena sp. infection in guppies (Poecilia reticulate). Fish Shellfish. Immunol. 27(2), 302–308 (2009).
Takahashi, Y. & Kawahara, E. Maternal immunity in newborn fry of the ovoviparous guppy. Nippon Suisan. Gakkaishi. 53(5), 721–725 (1987).
Leibowitz, M.P. et al. Cysteine proteases and acid phosphatases contribute to Tetrahymena spp. pathogenicity in guppies. Poecilia reticulata. Vet Parasitol. 166(1–2), 21–26 (2009).
Bretaud, S., Lee, S., & Guo, S. Sensitivity of zebrafish to environmental toxins implicated in Parkinson’s disease. Neurotoxicol. Teratol. 26(6), 857–864 (2004).
Uren-Webster, T.M. et al. Mechanisms of toxicity of di(2-ethylhexyl) phthalate on the reproductive health of male zebrafish. Aquat. Toxicol. 99(3), 360–369 (2010).
Liu, Y. et al. Induction of time-dependent oxidative stress and related transcriptional effects of perfluorododecanoic acid in zebrafish liver. Aquat. Toxicol. 89(4), 242–250 (2008).
Yin, N. et al. Effects of adrenergic agents on the expression of zebrafish (Danio rerio) vitellogenin Ao1. Toxicol. Appl. Pharmacol. 238(1), 20–26 (2009).
Ninkovic, J. & Bally-Cuif, L. The zebrafish as a model system for assessing the reinforcing properties of drugs of abuse. Methods 39(3), 262–274 (2006).
Hatefi, A. & Amsden, B. Biodegradable injectable in situ forming drug delivery systems. J. Control Release 80(1–3), 9–28 (2002).
de Bree, E. et al. Treatment of ovarian cancer using intraperitoneal chemotherapy with taxanes: from laboratory bench to bedside. Cancer Treat. Rev. 32(6), 471–482 (2006).
Wang, H.Y. et al. Localization and analyses of small drug molecules in rat brain tissue sections. Anal. Chem. 77(20), 6682–6686 (2005).
Toth, K. et al. Effects of intraamygdaloid microinjections of acylated-ghrelin on liquid food intake of rats. Brain Res. Bull. 77(2–3), 105–111 (2008).
DeTolla, L.J., et al. Guidelines for the care and use of fish in research. ILAR J. 37(4), 159–173 (1995).
Lopatin, P.V. et al. Use of nonaqueous solvents to prepare injection solutions. Khimiko-Farmatsevticheskii Zhurnal. 6(11), 36–47 (1973).
IACUC, Anesthesia, Analgesia and Euthanasia Guide, U.O.o. Research, 1995.
NIH, NIH Anesthesia/Analgesia Formulary. 2005.
Ayllon, F. & Garcia-Vazquez, E. Induction of micronuclei and other nuclear abnormalities in European minnow Phoxinus phoxinus and mollie Poecilia latipinna: an assessment of the fish micronucleus test. Mutat. Res. 467(2), 177–186 (2000).
Westerfield, M. (ed.) The zebrafish Book. A Guide for the Laboratory Use of Zebrafish (Danio rerio) (University of Oregon Press, Eugene, 2000).
Frisen, L. Reliability of intraperitoneal injections in fish. Experientia 23(10), 883–884 (1967).
Swaim, L.E. et al. Mycobacterium marinum infection of adult zebrafish causes caseating granulomatous tuberculosis and is moderated by adaptive immunity. Infect. Immun. 74(11), 6108–6117 (2006).
Neely, M.N., Pfeifer, J.D., & Caparon, M. Streptococcus-zebrafish model of bacterial pathogenesis. Infect. Immun. 70(7), 3904–3914 (2002).
Lien, C.L. et al. Gene expression analysis of zebrafish heart regeneration. PLoS Biol. 4(8), e260 (2006).
Novoa, A. et al. Zebrafish (Danio rerio) as a model for the study of vaccination against viral haemorrhagic septicemia virus (VHSV.) Vaccine 24(31–32), 5806–5816 (2006).
Moss, J.B. et al. Regeneration of the pancreas in adult zebrafish. Diabetes 58(8), 1844–1851 (2009).
Pugach, E.K., et al. Retro-orbital injection in adult zebrafish. J. Vis. Exp. 12(34), 1–2 (2009).
Braida, D. et al. Hallucinatory and rewarding effect of salvinorin A in zebrafish: kappa-opioid and CB1-cannabinoid receptor involvement. Psychopharmacology (Berlin) 190(4), 441–448 (2007).
Levina, S. & Gordon, R. Methionine enkephalin-induced changes in pigmentation of zebrafish (Cyprinidae, Brachydanio rerio) and related species and varieties, measured videodensitometrically. I. Zebrafish. Gen. Comp. Endocrinol. 51(3), 370–377 (1983).
Anichtchik, O.V., et al. Neurochemical and behavioural changes in zebrafish Danio rerio after systemic administration of 6-hydroxydopamine and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. J. Neurochem. 88(2), 443–453 (2004).
Lord, A.M., North, T.E., & Zon, L.I. Prostaglandin E2: making more of your marrow. Cell Cycle 6(24), 3054–3057 (2007).
Lee, S.J. et al. LPA1 is essential for lymphatic vessel development in zebrafish. FASEB J. 22(10), 3706–3715 (2008).
Hornberg, T.E. Experimental methods for pharmacokinetic studies in salmonids. Ann. Rev. Fish Diseases 4, 345–358 (1994).
Green, M.D. & Lomax, P. Behavioral thermoregulation and neuroamines in fish (Chromus chromus). J. Thermal Biol. 1(4), 237–240 (1976).
Sutphin, Z.A., Myrick, C.A., & Brandt, M.M. Swimming performance of sacramento splittail injected with subcutaneous marking agents. N. Amer. J. Fisheries Manage. 27, 1378–1382 (2007).
Thompson, E.R. et al. Induction of bioluminescence capability in the marine fish Porichthys notatus, by vargula (crustacean) [14C]luciferin and unlabelled analogues. J. Exp. Biol. 137, 39–51 (1988).
Cachat, J.M. et al. Measuring behavioral and endocrine responses to novelty stress in adult zebrafish. Nat. Protocols, in press (2010).
Acknowledgments
The study was supported by Tulane university Intramural funds, the Gordon and the G. Lurcy Fellowships, Provost’s Scholarly Enrichment Fund, Newcomb Fellows Grant, LA Board of Regents Pfund, NARSAD YI award and Zebrafish Neuroscience Research Consortium (ZNRC).
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Stewart, A. et al. (2011). Intraperitoneal Injection as a Method of Psychotropic Drug Delivery in Adult Zebrafish. In: Kalueff, A., Cachat, J. (eds) Zebrafish Neurobehavioral Protocols. Neuromethods, vol 51. Humana Press. https://doi.org/10.1007/978-1-60761-953-6_14
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DOI: https://doi.org/10.1007/978-1-60761-953-6_14
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