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Defense and Counterdefense in the RNAi-Based Antiviral Immune System in Insects

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Antiviral RNAi

Part of the book series: Methods in Molecular Biology ((MIMB,volume 721))

Abstract

RNA interference (RNAi) is an important pathway to combat virus infections in insects and plants. Hallmarks of antiviral RNAi in these organisms are: (1) an increase in virus replication after inactivation of major actors in the RNAi pathway, (2) production of virus-derived small interfering RNAs (v-siRNAs), and (3) suppression of RNAi by dedicated viral proteins. In this chapter, we will review the mechanism of RNAi in insects, its function as an antiviral immune system, viral small RNA profiles, and viral counterdefense strategies. We will also consider alternative, inducible antiviral immune responses.

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Acknowledgments

This work was financially supported by a fellowship from the Nijmegen Center for Molecular Life Sciences, by a VIDI fellowship from the Netherlands Organization for Scientific Research (project number 864.08.003), and by Horizon Breakthrough fellowships from the Netherlands Genomics Initiative (project numbers 93519018 and 93518020).

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  1. Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, Nijmegen Centre for Molecular Life Sciences, Nijmegen Institute for Infection, Inflammation and Immunity, Nijmegen, The Netherlands

    Ronald P. van Rij

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  1. Joël T. van Mierlo
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  2. Koen W. R. van Cleef
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  3. Ronald P. van Rij
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Correspondence to Ronald P. van Rij .

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  1. Inst. for Infection, Inflammation & Immu, Dept. Medical Microbiology, Radboud Univ.Nijmegen Medical Ctr, Geert Grooteplein 28, Nijmegen, 6525 GA, Netherlands

    Ronald P. van Rij

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van Mierlo, J.T., van Cleef, K.W.R., van Rij, R.P. (2011). Defense and Counterdefense in the RNAi-Based Antiviral Immune System in Insects. In: van Rij, R. (eds) Antiviral RNAi. Methods in Molecular Biology, vol 721. Humana Press. https://doi.org/10.1007/978-1-61779-037-9_1

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  • DOI: https://doi.org/10.1007/978-1-61779-037-9_1

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-036-2

  • Online ISBN: 978-1-61779-037-9

  • eBook Packages: Springer Protocols

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