Abstract
WST-8 is one of the newer generation formazan-based dyes, which release the converted product into the medium in a soluble form. This allows for a non-destructive determination of viability enabling the cells to be subject to further investigations. This is a major advantage in cases where cell phenotype is being investigated and data such as matrix synthesis is correlated to cell number. This chapter describes the use of WST-8 to normalise alkaline phosphatase activity.
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References
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Stoddart, M.J. (2011). WST-8 Analysis of Cell Viability During Osteogenesis of Human Mesenchymal Stem Cells. In: Stoddart, M. (eds) Mammalian Cell Viability. Methods in Molecular Biology, vol 740. Humana Press. https://doi.org/10.1007/978-1-61779-108-6_4
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DOI: https://doi.org/10.1007/978-1-61779-108-6_4
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Publisher Name: Humana Press
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Online ISBN: 978-1-61779-108-6
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