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Positional Proteomics at the N-Terminus as a Means of Proteome Simplification

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Gel-Free Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 753))

Abstract

One strategy to reduce complexity in proteome analysis is through rational reduction of the proteolytic peptides that constitute the analyte for mass spectrometric analysis. Methods for selective isolation of C- and N-terminal peptides have been developed. In this chapter, we outline the context and variety of methods for selective isolation of N-terminal peptides and detail one method based on negative selection through differential removal of internal peptides.

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Authors and Affiliations

  1. Protein Function Group, Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZJ, UK

    Gemma R. Davidson, Stuart D. Armstrong & Robert J. Beynon

Authors
  1. Gemma R. Davidson
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  2. Stuart D. Armstrong
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  3. Robert J. Beynon
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Corresponding author

Correspondence to Robert J. Beynon .

Editor information

Editors and Affiliations

  1. , VIB Department of Medical Protein Resear, VIB and Ghent University, Albert Baertsoenkaai 3, Gent, 9000, Belgium

    Kris Gevaert

  2. , VIB Department of Medical Protein Resear, VIB and Ghent University, Albert Baertsoenkaai 3, Gent, 9000, Belgium

    Joël Vandekerckhove

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Davidson, G.R., Armstrong, S.D., Beynon, R.J. (2011). Positional Proteomics at the N-Terminus as a Means of Proteome Simplification. In: Gevaert, K., Vandekerckhove, J. (eds) Gel-Free Proteomics. Methods in Molecular Biology, vol 753. Humana Press. https://doi.org/10.1007/978-1-61779-148-2_15

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  • DOI: https://doi.org/10.1007/978-1-61779-148-2_15

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-147-5

  • Online ISBN: 978-1-61779-148-2

  • eBook Packages: Springer Protocols

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