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Microglia Cell Culture: A Primer for the Novice

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In Vitro Neurotoxicology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 758))

Abstract

Microglial cells are the resident immune cells of the central nervous system. Progress in the recent decade has clearly established that microglial cells participate or even actively drive neurological disease. Much of our current knowledge has been generated by investigating microglial cells in cell culture. The aim of this chapter is to give the uninitiated a basic and adaptable protocol for the culturing of microglial cells. We discuss the challenges of microglial cell culture and provide a collection of tips which reflect our 25+ years of collective experience.

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Author information

Authors and Affiliations

  1. Department of Neurology and Center for Neurogenetics and Neurotherapeutics, University of Washington, Seattle, WA, USA

    Thomas Möller

Authors
  1. Anke Witting
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  2. Thomas Möller
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Corresponding author

Correspondence to Thomas Möller .

Editor information

Editors and Affiliations

  1. , Department of Environmental and, University of Washington, Roosevelt Way NE 4225, Seattle, 98105, Washington, USA

    Lucio G. Costa

  2. , Department of Environmental and, University of Washington, Roosevelt Way NE 4225, Seattle, 98105, Washington, USA

    Gennaro Giordano

  3. Jesse Brown VA Medical Center, Department of Psychiatry, University of Illinois at Chicago, South Damen Avenue 820, Seattle, 60612, Illinois, USA

    Marina Guizzetti

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Witting, A., Möller, T. (2011). Microglia Cell Culture: A Primer for the Novice. In: Costa, L., Giordano, G., Guizzetti, M. (eds) In Vitro Neurotoxicology. Methods in Molecular Biology, vol 758. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-170-3_4

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  • DOI: https://doi.org/10.1007/978-1-61779-170-3_4

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-169-7

  • Online ISBN: 978-1-61779-170-3

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