Abstract
Transcription factors regulate transcription by binding to regulatory regions of genes including the promoter. Few of the transcription factors are well characterized, and few promoters have been described in detail. New methods have been developed to improve both transcription factor and promoter characterization, some of which are discussed here. Trapping methodology applicable to both individual transcription factors and intact transcription complexes are described, as well as 2D gel electrophoresis, Southwestern blotting, and basic liquid chromatography/tandem mass spectrometry methodology. These methods have proved useful in the study of transcriptional regulation.
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Acknowledgments
We thank Dr. William Haskins and the UTSA RCMI Proteomics and Protein Biomarkers Core facility for help with analysis and the use of their equipment. We also appreciate the help of UTSA RCMI Bioinformatics and Computational Biology core for computing. The RCMI facilities are supported by grant 2G12RR013646 from the NIH. We also thank Daifang Jiang for reading the manuscript and for much helpful discussion. This work was supported by NIH grant R01GM043609 and a grant from the San Antonio Life Sciences Institute.
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Jarrett, H.W. (2012). Proteomic Methodologies to Study Transcription Factor Function. In: Deplancke, B., Gheldof, N. (eds) Gene Regulatory Networks. Methods in Molecular Biology, vol 786. Humana Press. https://doi.org/10.1007/978-1-61779-292-2_19
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DOI: https://doi.org/10.1007/978-1-61779-292-2_19
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