Abstract
We present here a method for construction of carbohydrate cluster microarrays. This technology utilizes a 3-dimensional poly(amidoamine) starburst dendrimer monolayer assembled on glass surface, which is functionalized with terminal hydrazide groups for site-specific coupling of carbohydrates without prior chemical derivatization. Microwave radiation energy is applied to accelerate carbohydrate coupling on chips. Since this bioarray platform is designed to present sugar chains in defined orientation and cluster configurations, it is particularly suitable for exploration of the structural and conformational diversities of glycoepitopes and their functional properties.
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References
Paulson, J. C., Blixt, O., Collins, B. E. (2006) Sweet spots in functional glycomics. Nat. Chem. Biol. 2, 238–248.
Wang, D. (2003) Carbohydrate microarrays. Proteomics, 3, 2167–2175.
Feizi, T., Chai, W. (2004) Oligosaccharide microarrays to decipher the glyco-code. Nat. Rev. Mol. Cell Biol. 5, 582–588.
Park, S., Lee, M. R., Shin, I. (2008) Carbohydrate microarrays as powerful tools in studies of carbohydrate-mediated biological processes. Chem. Commun. 374389–4399.
Wang, D., Liu, S., Trummer, B. J., Deng, C., Wang, A. (2002) Carbohydrate microarrays for the recognition of cross-reactive molecular markers of microbes and host cells. Nat. Biotechnol. 20, 275–281.
Wang, D., Lu, J. (2004) Glycan arrays lead to the discovery of autoimmunogenic activity of SARS-CoV. Physiol. Genomics 18, 245–248.
Willats,W. G. T., Rasmussen, S. E., Kristensen, T., Mikkelsen, J. D., Knox, J. P. (2002) Sugar-coated microarrays: a novel slide surface for the high-throughput analysis of glycans. Proteomics. 2, 1666–1671.
Xia, B., Kawar, Z. S., Ju, T., Alvarez, R. A., Sachdev, G. P., Cummings, R. D. (2005) Versatile fluorescent derivatization of glycans for glycomic analysis. Nat. Methods 2, 845–850.
Blixt, O., Head, S., Mondala, T., Scanlan, C., Huflejt, M. E., Alvarez, R., Bryan, M. C., Fazio, F., Calarese, D., Stevens, J., Razi, N., Stevens, D. J., Skehel, J. J., van Die, I., Burton, D. R., Wilson, I. A., Cummings, R., Bovin, N., Wong, C. H., Paulson, J. C. (2004) Printed covalent glycan array for ligand profiling of diverse glycan binding proteins. Proc. Natl. Acad. Sci. U.S.A. 101, 17033–17038.
Ratner, D. M., Adams, E. W., Su, J., O’Keefe, B. R., Mrksich, M., Seeberger, P. H. (2004) Probing Protein-Carbohydrate Interactions with Microarrays of Synthetic Oligosaccharides. ChemBioChem 5, 379–382.
Houseman, B. T., Mrksich, M. (2002) Carbohydrate Arrays for the Evaluation of Protein Binding and Enzyme Activity. Chem. Biol. 9, 443–454.
Fukui, S., Feizi, T., Galustian, C., Lawson, A. M., Chai, W. (2002) Oligosaccharide microarrays for high-throughput detection and specificity assignments of carbohydrate-protein interactions. Nat. Biotechnol. 201011–1017.
Pohl, N. L. (2008) Fluorous Tags Catching on Microarrays. Angew. Chem., Int. Ed. 47, 3868–3870.
Sun, X. L., Stabler, C. L., Cazalis, C. S., Chaikof, E. L. (2006) Carbohydrate and protein immobilization onto solid surfaces by sequential Diels-Alder and azide-alkyne cycloadditions. Bioconjugate Chem. 17, 52–57.
Kohn, M., Wacker, R., Peters, C., Schroder, H., Soulere, L., Breinbauer, R., Niemeyer, C., Waldmann, H. (2003) Staudinger ligation: a new immobilization strategy for the preparation of small-molecule arrays. Angew. Chem., Int. Ed. 42, 5830–5834.
Feizi, T., Fazio, F., Chai, W. Wong, C. H. (2003). Carbohydrate microarrays - a new set of technologies at the frontiers of glycomics. Curr Opin Struct Biol. 13, 637–645.
Carroll, G. T., Wang, D., Turro, N. J., Koberstein, J. T., Photochemical micropatterning of carbohydrates on a surface. Langmuir 2006 22, (6), 2899–905.
Wang, D., Carroll, G. T., Turro, N. J., Koberstein, J. T., Kovac, P., Saksena, R., Adamo, R., Herzenberg, L. A., Herzenberg, L. A., Steinman, L., Photogenerated glycan arrays identify immunogenic sugar moieties of Bacillus anthracis exosporium. Proteomics 2007 7, (2), 180–4.
Acknowledgments
This work was supported by Grant Number 1R43 RR023763-01 and 2R44 RR023763-02 (X.C. Zhou) from the National Center for Research Resources Services (NCRR) and U01CA128416 (D. Wang) from National Cancer Institute (NCI), components of the National Institutes of Health (NIH). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NCRR or NCI or the National Institutes of Health.
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Zhou, X., Zhang, J., Wang, D. (2012). Microwave-Assisted Method for Fabrication of Carbohydrate Cluster Microarrays on 3-Dimensional Hydrazide–Dendrimer Substrate. In: Chevolot, Y. (eds) Carbohydrate Microarrays. Methods in Molecular Biology, vol 808. Humana Press. https://doi.org/10.1007/978-1-61779-373-8_27
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DOI: https://doi.org/10.1007/978-1-61779-373-8_27
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