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mRNA Display Using Covalent Coupling of mRNA to Translated Proteins

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Ribosome Display and Related Technologies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 805))

Abstract

mRNA display is a powerful technique that allows for covalent coupling of a translated protein with its coding mRNA. The resulting conjugation between genotype and phenotype can be used for the efficient selection and identification of peptides or proteins with desired properties from an mRNA-displayed peptide or protein library with high diversity. This protocol outlines the principle of mRNA display and the detailed procedures for the synthesis of mRNA–protein fusions. Some special considerations for library construction, generation, and purification are discussed.

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Authors and Affiliations

  1. Eshelman School of Pharmacy and Carolina Center for Genome Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

    Rong Wang, Steve W. Cotten & Rihe Liu

Authors
  1. Rong Wang
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  2. Steve W. Cotten
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  3. Rihe Liu
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Corresponding author

Correspondence to Rihe Liu .

Editor information

Editors and Affiliations

  1. , Antibody Discovery & Protein Engineering, MedImmune Limited, Granta Park, Cambridge, CB21 6GH, United Kingdom

    Julie A. Douthwaite

  2. , Antibody Discovery & Protein Engineering, MedImmune Limited, Granta Park, Cambridge, CB21 6GH, United Kingdom

    Ronald H. Jackson

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© 2012 Springer Science+Business Media, LLC

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Wang, R., Cotten, S.W., Liu, R. (2012). mRNA Display Using Covalent Coupling of mRNA to Translated Proteins. In: Douthwaite, J., Jackson, R. (eds) Ribosome Display and Related Technologies. Methods in Molecular Biology, vol 805. Springer, New York, NY. https://doi.org/10.1007/978-1-61779-379-0_6

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  • DOI: https://doi.org/10.1007/978-1-61779-379-0_6

  • Published:

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-61779-378-3

  • Online ISBN: 978-1-61779-379-0

  • eBook Packages: Springer Protocols

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