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Genetic Engineering of Industrial Strains of Saccharomyces cerevisiae

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Recombinant Gene Expression

Part of the book series: Methods in Molecular Biology ((MIMB,volume 824))

Abstract

Genetic engineering has been successfully applied to Saccharomyces cerevisiae laboratory strains for different purposes: extension of substrate range, improvement of productivity and yield, elimination of by-products, improvement of process performance and cellular properties, and extension of product range. The potential of genetically engineered yeasts for the massive production of biofuels as bioethanol and other nonfuel products from renewable resources as lignocellulosic biomass hydrolysates has been recognized. For such applications, robust industrial strains of S. cerevisiae have to be used. Here, some relevant genetic and genomic characteristics of industrial strains are discussed in relation to the problematic of the genetic engineering of such strains. General molecular tools applicable to the manipulation of S. cerevisiae industrial strains are presented and examples of genetically engineered industrial strains developed for the production of bioethanol from lignocellulosic biomass are given.

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Authors and Affiliations

  1. Departamento de Procesos y Tecnología, Universidad Autónoma Metropolitana-Cuajimalpa, Mexico City, Mexico

    Sylvie Le Borgne

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  1. Sylvie Le Borgne
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Correspondence to Sylvie Le Borgne .

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Editors and Affiliations

  1. Dept. Chemistry & Physics, Arkansas State University, Jonesboro, University Loop E. 504, State University, 72401, Arkansas, USA

    Argelia Lorence

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Le Borgne, S. (2012). Genetic Engineering of Industrial Strains of Saccharomyces cerevisiae . In: Lorence, A. (eds) Recombinant Gene Expression. Methods in Molecular Biology, vol 824. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-433-9_24

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  • DOI: https://doi.org/10.1007/978-1-61779-433-9_24

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-432-2

  • Online ISBN: 978-1-61779-433-9

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