Abstract
Microarray expression profiling of the nervous system provides a powerful approach to ascribe activities to genes involved in distinct phases of neural development and function. Expression profiling of neural tissues and cell lines requires isolation of high-quality RNA, amplification of the isolated RNA, and hybridization to DNA microarrays. In this chapter, theoretical background for expression profiling as well as protocols for reproducible microarray experiments from brain tissue and cell lines derived from brain tissue will be presented in the context of neural proliferation and tumorigenesis in the cerebellum.
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Acknowledgments
This work was supported by grants to E. D. from the Alfred P. Sloan Research Foundation, the James S. McDonnell Foundation Twenty-first Century Award Program, the UC Davis Health System Research Award Program, an individual allocation of the UC Davis Institutional Research Grant from the American Cancer Society, a Career Development Award from the UCSF Brain Tumor SPORE Program, and a NIH Director’s New Innovator Award. G. A. B. was supported in part by a postdoctoral fellowship from the California Institute of Regenerative Medicine.
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Barisone, G.A., Díaz, E. (2012). DNA Microarrays for Gene Expression Analysis in Brain Tissue and Cell Lines. In: Karamanos, Y. (eds) Expression Profiling in Neuroscience. Neuromethods, vol 64. Humana Press. https://doi.org/10.1007/978-1-61779-448-3_1
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DOI: https://doi.org/10.1007/978-1-61779-448-3_1
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