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Analysis of Single Eukaryotic Cells Using Raman Tweezers

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Single-Cell Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 853))

Abstract

Raman Tweezers is a technique that combines optical trapping with Raman spectroscopy and has enabled the spectroscopic analysis of single cells. Applications of this technique include the identification and discrimination of different types of cells, including healthy and non-healthy cells (e.g. cancer cells). In addition, the interaction of cells with stimuli, e.g. drugs, can also be studied on a single-cell basis. Herein, a generic protocol for the analysis of fixed and living single eukaryotic cells is described, including the considerations required to build a Raman Tweezers systems.

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Author information

Authors and Affiliations

  1. Manchester Interdisciplinary Biocentre, School of Chemical Engineering and Analytical Science, The University of Manchester, Manchester, UK

    Elsa Correia Faria & Peter Gardner

Authors
  1. Elsa Correia Faria
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  2. Peter Gardner
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Corresponding author

Correspondence to Elsa Correia Faria .

Editor information

Editors and Affiliations

  1. POOLIA Sweden, Warfvinges väg 20, Stockholm, 104 25, Sweden

    Sara Lindström

  2. , Division of Nanobiotechnology, Royal Institute of Technology, Roslagstullsbacken 21, Stockholm, 106 91, Sweden

    Helene Andersson-Svahn

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Faria, E.C., Gardner, P. (2012). Analysis of Single Eukaryotic Cells Using Raman Tweezers. In: Lindström, S., Andersson-Svahn, H. (eds) Single-Cell Analysis. Methods in Molecular Biology, vol 853. Humana Press. https://doi.org/10.1007/978-1-61779-567-1_12

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  • DOI: https://doi.org/10.1007/978-1-61779-567-1_12

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-566-4

  • Online ISBN: 978-1-61779-567-1

  • eBook Packages: Springer Protocols

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