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Assessment of Neurogenesis in Models of Focal Cerebral Ischemia

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Animal Models of Acute Neurological Injuries II

Part of the book series: Springer Protocols Handbooks ((SPH))

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Abstract

Neurogenesis is the generation of new neurons. In hippocampal dentate gyrus and olfactory bulb, which usually are referred to as the neurogenic region in the mammalian brain, new neurons are developed daily throughout their lifetime. In the non-neurogenic region of the brain, neurogenesis occurs only under certain pathological conditions. Focal cerebral ischemia is a common neurological disease that stimulates neurogenesis in both neurogenic and non-neurogenic regions in adult brains. The integration between the preexisting and the newly developed neurons in the ischemic regions happens after the new ones matured morphologically and functionally. This chapter introduces the methods of studying neurogenesis using focal cerebral ischemia models, including morphological analysis by performing immunohistochemical and immunofluorescent multiple staining with confocal scanning and immunoelectron microscopy, and functional determination of neurons by using electrophysiological recording and FM dye synaptic vesicle staining in animal brain slices containing retroviral green fluorescent protein-labeled new neurons.

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Acknowledgments

This work was supported by grants from National Basic Research Program of China (2006CB943702 and 2006CB504100) and National Nature Science Foundation of China (81030020 and 30770660).

Author information

Authors and Affiliations

  1. Department of Neurobiology and State Key Laboratory of Medical Neurobiology, Shanghai Medical College Fudan University, Shanghai, China

    Feng-Yan Sun

  2. Institutes for Biomedical Sciences, Fudan University, Shanghai, China

    Feng-Yan Sun

  3. Department of Neurobiology and State Key Laboratory of Medical Neurobiology, Shanghai Medical College Fudan University, Shanghai, People’s Republic of China

    Xiao Sun

  4. Department of Neurobiology and State Key Laboratory of Medical Neurobiology, Shanghai Medical College, Shanghai, People’s Republic of China

    Ji-Jiang Wang & Qiu-Wan Zhang

  5. Department of Neurobiology and State Key Laboratory of Medical Neurobiology, Shanghai Medical College, Shanghai, Shanghai, People’s Republic of China

    Yong-Quan Wang

Authors
  1. Feng-Yan Sun
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  2. Xiao Sun
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  3. Ji-Jiang Wang
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  4. Qiu-Wan Zhang
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  5. Yong-Quan Wang
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Corresponding author

Correspondence to Feng-Yan Sun .

Editor information

Editors and Affiliations

  1. School of Medicine, University of Pittsburgh, Terrace St. 3500, Pittsburgh, 15213, Pennsylvania, USA

    Jun Chen

  2. School of Medicine, Stark Neurosciences Res. Inst., Indiana University, W. Walnut St. 950, Indianapolis, 46202, Indiana, USA

    Xiao-Ming Xu

  3. School of Medicine, Department of Anatomy & Cell Biology, Indiana University, Barnhill Dr. 635, Indianapolis, 46202, Indiana, USA

    Zao C. Xu

  4. School of Medicine, Dept. Neurosurgery, Loma Linda University, Anderson St. 11234, Loma Linda, 92354, California, USA

    John H. Zhang

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Sun, FY., Sun, X., Wang, JJ., Zhang, QW., Wang, YQ. (2012). Assessment of Neurogenesis in Models of Focal Cerebral Ischemia. In: Chen, J., Xu, XM., Xu, Z., Zhang, J. (eds) Animal Models of Acute Neurological Injuries II. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-61779-782-8_17

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  • DOI: https://doi.org/10.1007/978-1-61779-782-8_17

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-781-1

  • Online ISBN: 978-1-61779-782-8

  • eBook Packages: Springer Protocols

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