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Methods for Cancer Stem Cell Detection and Isolation

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Somatic Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 879))

Abstract

The study and investigation of cancer stem cells (CSCs) or tumour initiating cells (TICs) have received enormous attention over the 10 years. CSCs are rare, quiescent and capable of self-renewing and maintaining tumour growth and heterogeneity. Better understanding of CSCs will no doubt lead to a new era of both basic and clinical cancer research, reclassification of human tumours and development of novel therapeutic strategies. Therefore, the biological properties of CSCs, the relevance of CSCs to cancer therapy and methodologies to identify them are essential in order to address real and efficacious therapeutic strategies to eradicate the cancer. Here, we describe the main protocols to identify CSCs starting from primary tumours including glioblastoma, sarcoma, lung and breast cancers.

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Author information

Authors and Affiliations

  1. Department of Experimental Medicine, Second University of Naples, Naples, Italy

    Virginia Tirino, Vincenzo Desiderio, Francesca Paino & Gianpaolo Papaccio

  2. Department of Experimental Medicine, Section of Biotechnology and Molecular Biology, Second University Of Naples, Naples, Italy

    Mario De Rosa

Authors
  1. Virginia Tirino
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  2. Vincenzo Desiderio
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  3. Francesca Paino
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  4. Gianpaolo Papaccio
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  5. Mario De Rosa
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Corresponding author

Correspondence to Gianpaolo Papaccio .

Editor information

Editors and Affiliations

  1. Mouse Cancer Genetics Program, National Cancer Institute, Center Drive 31, Frederick, 21702, Maryland, USA

    Shree Ram Singh

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© 2012 Springer Science+Business Media, LLC

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Tirino, V., Desiderio, V., Paino, F., Papaccio, G., De Rosa, M. (2012). Methods for Cancer Stem Cell Detection and Isolation. In: Singh, S. (eds) Somatic Stem Cells. Methods in Molecular Biology, vol 879. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-815-3_32

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  • DOI: https://doi.org/10.1007/978-1-61779-815-3_32

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-814-6

  • Online ISBN: 978-1-61779-815-3

  • eBook Packages: Springer Protocols

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