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Protocol for Gene Expression Profiling Using DNA Microarrays in Neisseria gonorrhoeae

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Diagnosis of Sexually Transmitted Diseases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 903))

Abstract

Gene expression profiling using DNA microarrays has become commonplace in current molecular biology practices, and has dramatically enhanced our understanding of the biology of Neisseria spp., and the interaction of these organisms with the host. With the choice of microarray platforms offered for gene expression profiling and commercially available arrays, investigators must ask several central questions to make decisions based on their research focus. Are arrays on hand for their organism and if not then would it be cost-effective to design custom arrays. Other important considerations; what types of specialized equipment for array hybridization and signal detection are required and is the specificity and sensitivity of the array adequate for your application. Here, we describe the use of a custom 12K CombiMatrix ElectraSense™ oligonucleotide microarray format for assessing global gene expression profiles in Neisseria spp.

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Author information

Authors and Affiliations

  1. Department of Microbiology and Immunology, The University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA

    Lydgia A. Jackson & David W. Dyer

Authors
  1. Lydgia A. Jackson
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  2. David W. Dyer
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Correspondence to Lydgia A. Jackson .

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Editors and Affiliations

  1. University Hospital, Institute of Medical Microbiology, Heinrich-Heine-University, Universitätssraße 1, Düsseldorf, 40225, Germany

    Colin R. MacKenzie

  2. University Hospital, Institute of Medical Microbiology, Heinrich-Heine-University, Universitätssraße 1, Düsseldorf, 40225, Germany

    Birgit Henrich

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Jackson, L.A., Dyer, D.W. (2012). Protocol for Gene Expression Profiling Using DNA Microarrays in Neisseria gonorrhoeae . In: MacKenzie, C., Henrich, B. (eds) Diagnosis of Sexually Transmitted Diseases. Methods in Molecular Biology, vol 903. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-937-2_24

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  • DOI: https://doi.org/10.1007/978-1-61779-937-2_24

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-936-5

  • Online ISBN: 978-1-61779-937-2

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