Abstract
The interaction of plants with their environment is very dynamic. Studying the underlying processes is important for understanding and modeling plant response to changing environmental conditions. Photosynthesis varies largely between different plants and at different locations within a canopy of a single plant. Thus, continuous and spatially distributed monitoring is necessary to assess the dynamic response of photosynthesis to the environment. Limited scale of observation with portable instrumentation makes it difficult to examine large numbers of plants under different environmental conditions. We report here on the application of a recently developed technique, laser-induced fluorescence transient (LIFT), for continuous remote measurement of photosynthetic efficiency of selected leaves at a distance of up to 50 m. The ability to make continuous, automatic, and remote measurements of photosynthetic efficiency of leaves with the LIFT provides a new approach for studying the interaction of plants with the environment and may become an important tool in phenotyping photosynthetic properties in field applications.
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References
Papageorgiou GC, Govindjee (2005) Chlorophyll a fluorescence: a signature of photosynthesis. Springer, Heidelberg
Genty B, Briantais JM, Baker NR (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim Biophys Acta 990:87–92
Schreiber U, Bilger W (1993) Progress in chlorophyll fluorescence research: major developments during the past years in retrospect. Prog Bot 54:151–173
Maxwell K, Johnson GN (2000) Chlorophyll fluorescence—a practical guide. J Exp Bot 51:659–668
Badger MR, Fallahi H, Kaines S et al (2009) Chlorophyll fluorescence screening of Arabidopsis thaliana for CO(2) sensitive photorespiration and photoinhibition mutants. Funct Plant Biol 36:867–873
Jansen M, Gilmer F, Biskup B et al (2009) Simultaneous phenotyping of leaf growth and chlorophyll fluorescence via GROWSCREEN FLUORO allows detection of stress tolerance in Arabidopsis thaliana and other rosette plants. Funct Plant Biol 36:902–914
Apostol S, Briantais JM, Moise N et al (2001) Photoinactivation of the photosynthetic electron transport chain by accumulation of over-saturating light pulses given to dark adapted pea leaves. Photosynth Res 67:215–227
Kolber Z, Prasil O, Falkowski PG (1998) Measurements of variable chlorophyll fluorescence using fast repetition rate techniques: defining methodology and experimental protocols. Biochim Biophys Acta 1367:88–106
Kolber Z, Klimov D, Ananyev G et al (2005) Measuring photosynthetic parameters at a distance: laser induced fluorescence transient (LIFT) method for remote measurement of photosynthesis in terrestrial vegetation. Photosynth Res 84:121–129
Ananyev G, Kolber Z, Klimov D et al (2005) Remote sensing of heterogeneity in photosynthetic efficiency, electron transport and dissipation of excess light in Populus deltoides stands under ambient and elevated CO2 concentrations, and in a tropical forest canopy, using a new laser-induced fluorescence transient device. Global Change Biol 11:1195–1206
Pieruschka R, Klimov D, Kolber Z et al (2010) Continuous measurements of the effects of cold stress on photochemical efficiency using laser induced fluorescence transient (LIFT) approach. Funct Plant Biol 37:395–402
Rascher U, Pieruschka R (2008) Spatio-temporal variations of photosynthesis: the potential of optical remote sensing to better understand and scale light use efficiency and stresses of plant ecosystems. Prec Agric 9:355–366
Laisk A, Loreto F (1996) Determining photosynthetic parameters from leaf CO2 exchange and chlorophyll fluorescence. Plant Physiol 110:903–912
Peterson RB, Havir EA (2004) The multiphasic nature of nonphotochemical quenching: implications for assessment of photosynthetic electron transport based on chlorophyll fluorescence. Photosynth Res 82:95–107
Pieruschka R, Rascher U, Klimov D et al (2009) Optical remote sensing and laser induced fluorescence transients (LIFT) to quantify the spatio-temporal functionality of plant canopies. Nova Acta Leopoldina 96:49–62
Acknowledgments
We are very grateful to numerous colleagues at the Biosphere II Laboratories, Carnegie Institution for Science and, Forschungszentrum Jülich for the extensive support in developing and testing the LIFT apparatus. RP was supported by Marie Curie Outgoing International Fellowships (Nr: 041060-LIFT).
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Pieruschka, R., Klimov, D., Berry, J.A., Osmond, C.B., Rascher, U., Kolber, Z.S. (2012). Remote Chlorophyll Fluorescence Measurements with the Laser-Induced Fluorescence Transient Approach. In: Normanly, J. (eds) High-Throughput Phenotyping in Plants. Methods in Molecular Biology, vol 918. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-995-2_5
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DOI: https://doi.org/10.1007/978-1-61779-995-2_5
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