Abstract
GABA (γ-aminobutyric acid)-ergic interneurons are a structurally and functionally diverse group of cells that, together, regulate the activity of neuronal networks giving rise to the brain oscillations necessary for information processing. The phenotypes of GABAergic neurons can be classified by morphological, electrophysiological, and neurochemical features. However, as further information is gathered about these features and the role(s) each phenotype plays in shaping cortical function, group membership will undoubtedly change. This chapter details a set of methodologies that can be used to characterize GABAergic neurons in the monkey dorsolateral prefrontal cortex (DLPFC). Specifically, it (1) provides a brief protocol for the dissection of DLPFC tissue from monkeys, (2) describes electrophysiological measures used to identify GABAergic neurons, (3) details how to fill neurons for morphological analyses, and (4) describes a fluorescence imaging technique for the quantification of fluorescently labeled puncta (putative synaptic components), the colocalization of different labels in the same synaptic structure, and the quantitation of fluorescence intensity in these same structures in brain tissue sections.
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Acknowledgments
This work was supported by the NIMH (MH051234 and MH084053 to DAL, MH085108 to KNF).
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Fish, K.N., Gonzalez-Burgos, G., Zaitsev, A.V., Lewis, D.A. (2012). Histological Characterization of Physiologically Determined Fast-Spiking Interneurons in Slices of Primate Dorsolateral Prefrontal Cortex. In: Ballanyi, K. (eds) Isolated Central Nervous System Circuits. Neuromethods, vol 73. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-020-5_4
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