Abstract
Ca2+-ATP pumps (those on the plasma membrane; PMCA and sarcoplasmic reticulum; SERCA1) have an important role to play in the regulation of intracellular calcium concentrations. In this chapter, three preparations, two membranes and a purified enzyme, best suited for studies of Ca2+-ATPase activity are described. The two selected membranes are the human red blood cell (RBC) ghosts, a representative of plasma membranes (PM), and the rabbit skeletal muscle SR, an intracellular membrane. In this protocol, Pi released during the ATPase reaction is subsequently measured colorimetrically as a complex of molybdovanadate. The method is simple (one-step), fast, sensitive, and reliable.
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Kosk-Kosicka, D. (2013). Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1). In: Lambert, D., Rainbow, R. (eds) Calcium Signaling Protocols. Methods in Molecular Biology, vol 937. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-086-1_21
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DOI: https://doi.org/10.1007/978-1-62703-086-1_21
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-085-4
Online ISBN: 978-1-62703-086-1
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