Abstract
Fungi produce an astonishing variety of secondary metabolites, some of which belong to the most toxic compounds in the living world. However, the benefits of secondary metabolites for fungi are often obscure. Here we describe a method that will aid in the analysis of fungal gene expression upon interkingdom competition with insects. It allows for isolation of fungal RNA from surface-grown mycelium after confrontation with insect larvae, and subsequent molecular analysis.
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References
Keller NP, Turner G, Bennett JW (2005) Fungal secondary metabolism - from biochemistry to genomics. Nat Rev Microbiol 3:937–947
Kempken F, Rohlfs M (2010) Fungal secondary metabolite biosynthesis - a chemical defense strategy against antagonistic animals? Fungal Ecol 3:107–114
Rohlfs M, Albert M, Keller NP, Kempken F (2007) Secondary chemicals protect mould from fungivory. Biol Lett 3:523–525
Rohlfs M, Trienens M, Fohgrub U, Kempken F (2009). Evolutionary and ecological interactions of moulds and insects. In: Anke T (ed) The mycota XV. Physiology and genetics: selected basic and applied aspects. Springer, Heidelberg, pp. 131–151
Avanzo P, Sabotic J, Anzlovar S, Popovic T, Leonardi A et al (2009) Trypsin-specific inhibitors from the basidiomycete Clitocybe nebularis with regulatory and defensive functions. Microbiology 155:3971–3981
Bleuler-Martínez S, Butschi A, Garbani M, Wälti MA, Wohlschlager T et al (2011) A lectin-mediated resistance of higher fungi against predators and parasites. Mol Ecol 20:3056–3070
Kempken F (2011) Fungal defences against animal antagonists – lectins & more. Mol Ecol 20:2876–2877
Bok JW, Balajee SA, Marr KA, Andes D, Nielsen KF et al (2005) LaeA, a regulator of morphogenetic fungal virulence factors. Eukaryot Cell 4:1574–1582
Bok JW, Keller NP (2004) LaeA, a regulator of secondary metabolism in Aspergillus spp. Eukaryot Cell 3:527–535
Bok JW, Noordermeer D, Kale SP, Keller NP (2006) Secondary metabolic gene cluster silencing in Aspergillus nidulans. Mol Microbiol 61:1636–1645
Rohlfs M (2005) Density-dependent insect-mold interactions: effects on fungal growth and spore production. Mycologia 97:996–1001
Ashburner M (1989) Drosophila, a laboratory handbook. Cold Spring Habor Laboratory Press, New York
Rohlfs M, Obmann B, Petersen R (2005) Competition with filamentous fungi and its implications for a gregarious life-style in insects living on ephemeral resources. Ecol Entomol 30:556–563
Talaat AM, Lyons R, Howard ST, Johnston SA (2004) The temporal expression profile of Mycobacterium tuberculosis infection in mice. Proc Natl Acad Sci U S A 101:4602–4607
Wölfle S, Trienens M, Rohlfs M (2009) Experimental evolution of resistance against a competing fungus in Drosophila melanogaster. Oecologia 161:781–790
Allgaier S, Weiland N, Hamad I, Kempken F (2010) Expression of ribonuclease A and ribonuclease N1 in the filamentous fungus Neurospora crassa. Appl Microbiol Biotechnol 85:1041–1049
Acknowledgement
Part of this work was supported by the Deutsche Forschungsgeme-inschaft by a grant to F.K. We thank Marko Rohlfs (Göttingen) for critical discussion and suggestions.
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Fohgrub, U., Kempken, F. (2012). Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects. In: Keller, N., Turner, G. (eds) Fungal Secondary Metabolism. Methods in Molecular Biology, vol 944. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-122-6_21
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DOI: https://doi.org/10.1007/978-1-62703-122-6_21
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Publisher Name: Humana Press, Totowa, NJ
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