Abstract
Identifying molecules that serve as markers for cell aging is a goal that has been pursued by several groups. Senescence-associated β galactosidase (SA-βgal) staining is broadly used and very easily detected. β-gal is a lysosomal enzyme strongly correlated to the progression of cell senescence. Here, we describe a simple, fast, and quantitative protocol to quantify SA-βgal activity in cell lysate extracts by a chemiluminescent method using galacton as substrate.
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References
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Bassaneze, V., Miyakawa, A.A., Krieger, J.E. (2013). Chemiluminescent Detection of Senescence-Associated β Galactosidase. In: Galluzzi, L., Vitale, I., Kepp, O., Kroemer, G. (eds) Cell Senescence. Methods in Molecular Biology, vol 965. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-239-1_9
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DOI: https://doi.org/10.1007/978-1-62703-239-1_9
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-238-4
Online ISBN: 978-1-62703-239-1
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