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Chiral Separations by HPLC on Immobilized Polysaccharide Chiral Stationary Phases

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Chiral Separations

Part of the book series: Methods in Molecular Biology ((MIMB,volume 970))

Abstract

Immobilized polysaccharide chiral stationary phases (CSPs) are a new development in chiral chromatography with increasing importance for the resolution of racemic compounds. These CSPs can be used with solvents traditionally applied for the classical coated polysaccharide CSPs as well as solvents that were “forbidden” for the coated phases such as tetrahydrofuran, chloroform, dichloromethane, acetone, 1,4-dioxane, ethylacetate, and certain ethers. However, these forbidden solvents may be required for the determination of the chiral recognition mechanism. Therefore, the immobilized CSPs are effective for the evaluation of chiral recognition mechanisms. Furthermore, immobilized chiral columns are also useful for monitoring the progress of stereospecific synthetic reactions which are normally performed in such solvents. The present chapter describes the detailed experimental protocol of a chiral resolution on immobilized polysaccharides CSPs by HPLC.

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Acknowledgments

Zeid A. AL-Othman would like to thank the Research Center, College of Science, King Saud University, for the financial support.

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Authors and Affiliations

  1. Department of Chemistry, Jamia Millia Islamia (Central University), New Delhi, India

    Imran Ali

  2. Department of Chemistry, King Saud University, Riyadh, Kingdom of Saudi Arabia

    Zeid A. AL-Othman

  3. National Pharmaceutical and Medicinal Chemistry Department, Research Centre, Cairo, Egypt

    Hassan Y. Aboul-Enein

Authors
  1. Imran Ali
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  2. Zeid A. AL-Othman
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  3. Hassan Y. Aboul-Enein
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Corresponding author

Correspondence to Hassan Y. Aboul-Enein .

Editor information

Editors and Affiliations

  1. , Department of Pharmaceutical Chemistry, Friedrich-Schiller-University Jena, Philosophenweg 14, Jena, 07743, Germany

    Gerhard K. E. Scriba

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Ali, I., AL-Othman, Z.A., Aboul-Enein, H.Y. (2013). Chiral Separations by HPLC on Immobilized Polysaccharide Chiral Stationary Phases. In: Scriba, G. (eds) Chiral Separations. Methods in Molecular Biology, vol 970. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-263-6_7

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  • DOI: https://doi.org/10.1007/978-1-62703-263-6_7

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-262-9

  • Online ISBN: 978-1-62703-263-6

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