Abstract
Pancreatic cancer remains a challenging disease, with an overall 5-year survival rate below 5%, the main reason being that it has an extremely high potential for invasion and metastasis. This potential may contribute to the fact that in more than three fourths of patients diagnosed with pancreatic cancer, it has already spread locally and to distant organs, precluding curative resection. Therefore, improved understanding of the mechanisms underlying pancreatic cancer metastasis is urgently needed. In this chapter, we describe our approaches to determining the metastatic potential of pancreatic cancer cells. Specifically, we report the details of these approaches, including in vitro assays of migration, invasion, adhesion, and angiogenesis and in vivo models of liver and lung metastasis and angiogenesis.
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Acknowledgments
We thank Don Norwood for editorial comments. This work is supported in part by grants R01-CA129956, R01-CA148954, and R01CA152309 (to K. Xie) from the National Cancer Institute, National Institutes of Health, and grant 81101844 (to C. Huang) from the National Natural Science Foundation of China.
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Huang, C., Xie, K. (2013). Analysis of the Potential for Pancreatic Cancer Metastasis In Vitro and In Vivo. In: Su, G. (eds) Pancreatic Cancer. Methods in Molecular Biology, vol 980. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-287-2_17
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DOI: https://doi.org/10.1007/978-1-62703-287-2_17
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-286-5
Online ISBN: 978-1-62703-287-2
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