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In Vitro Evolution of Enzymes

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Enzyme Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 978))

Abstract

In the past decade, in vitro evolution techniques have been used to improve the performance or alter the activity of a number of different enzymes and have generated enzymes de novo. In this review, we provide an overview of the available in vitro methods, their application, and some general considerations for enzyme engineering in vitro. We discuss the advantages of in vitro over in vivo approaches and focus on ribosome display, mRNA display, DNA display technologies, and in vitro compartmentalization (IVC) methods. This review aims to help researchers determine which approach is best suited for their own experimental needs and to highlight that in vitro methods offer a promising route for enzyme engineering.

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Authors and Affiliations

  1. Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, St. Paul, MN, USA

    Misha V. Golynskiy & John C. Haugner III

  2. BioTechnology Institute, University of Minnesota, St. Paul, MN, USA

    Misha V. Golynskiy & John C. Haugner III

  3. Department of Biochemistry, Molecular Biology and Biophysics & BioTechnology Institute, University of Minnesota, St. Paul, MN, USA

    Aleardo Morelli, Dana Morrone & Burckhard Seelig

Authors
  1. Misha V. Golynskiy
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  2. John C. Haugner III
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  3. Aleardo Morelli
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  4. Dana Morrone
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  5. Burckhard Seelig
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Corresponding author

Correspondence to Burckhard Seelig .

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Editors and Affiliations

  1. New England Biolabs, Inc., County Road 240, Ipswich, 01938, Massachusetts, USA

    James C. Samuelson

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Golynskiy, M.V., Haugner, J.C., Morelli, A., Morrone, D., Seelig, B. (2013). In Vitro Evolution of Enzymes. In: Samuelson, J. (eds) Enzyme Engineering. Methods in Molecular Biology, vol 978. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-293-3_6

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  • DOI: https://doi.org/10.1007/978-1-62703-293-3_6

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  • Print ISBN: 978-1-62703-292-6

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