Skip to main content
SpringerLink
Log in

Preparation and Characterization of DNA Block Copolymer Assemblies Loaded with Nanoparticles

  • Protocol
  • First Online:
Nanomaterial Interfaces in Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1025))

  • 3545 Accesses

Abstract

We have recently developed a universal procedure to functionalize inorganic nanoparticles with a dense layer of DNA through the self-assembly of DNA block copolymers and nanoparticles. This functionalization strategy allows one to combine the useful physical properties of inorganic nanoparticle with the enhanced DNA binding properties that originate from the high surface DNA density. In particular, the hybrid nanostructures exhibit orders of magnitude higher binding constants than regular DNA strands. This chapter presents a detailed protocol for the preparation and characterization of DNA block copolymer assemblies loaded with nanoparticles.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Abbreviations

CPG:

Controlled pore glass

DLS:

Dynamic light scattering

DMF:

N,N-Dimethylformamide

DNA-b-PS:

Block copolymer of DNA and polystyrene

FAM:

Fluorescein

FRET:

Förster/Fluorescent Resonance Energy Transfer

MNP:

Magnetic nanoparticles

MNP@PS@DNA:

DNA-b-polystyrene assemblies loaded with magnetic nanoparticles

PBS:

Phosphate buffer saline

PS:

Polystyrene

PS-MNP:

Polystyrene modified magnetic nanoparticles

PS@DNA:

Self assembly of DNA-b-polystyrene

PTFE:

Polytetrafluoroethylene

TEM:

Transmission electron microscope

THF:

Tetrahydrofuran

References

  1. Bardhan R, Lal S, Joshi A, Halas NJ (2011) Theranostic nanoshells: from probe design to imaging and treatment of cancer. Acc Chem Res 44:936–946

    Article  CAS  Google Scholar 

  2. Della Rocca J, Liu D, Lin W (2011) Nanoscale metal-organic frameworks for biomedical imaging and drug delivery. Acc Chem Res 44:957–968

    Article  CAS  Google Scholar 

  3. Xie J, Liu G, Eden HS, Ai H, Chen X (2011) Surface-engineered magnetic nanoparticle platforms for cancer imaging and therapy. Acc Chem Res 44:883–892

    Article  CAS  Google Scholar 

  4. Erathodiyil N, Ying JY (2011) Functionalization of inorganic nanoparticles for bioimaging applications. Acc Chem Res 44:925–935

    Article  CAS  Google Scholar 

  5. Yoo D, Lee J-H, Shin T-H, Cheon J (2011) Theranostic magnetic nanoparticles. Acc Chem Res 44:863–874

    Article  CAS  Google Scholar 

  6. Kievit FM, Zhang M (2011) Surface engineering of iron oxide nanoparticles for targeted cancer therapy. Acc Chem Res 44:853–862

    Article  CAS  Google Scholar 

  7. Chen X-J, Sanchez-Gaytan BL, Qian Z, Park S-J (2012) Noble metal nanoparticles in DNA detection and delivery. Wiley Interdiscip Rev Nanomed Nanobiotechnol 4:273–290

    Article  CAS  Google Scholar 

  8. Rosi NL, Mirkin CA (2005) Nanostructures in biodiagnostics. Chem Rev 105:1547–1562

    Article  CAS  Google Scholar 

  9. Chen X-J, Sanchez-Gaytan BL, Hayik SEN, Fryd M, Wayland BB, Park S-J (2010) Self-assembled hybrid structures of DNA block-copolymers and nanoparticles with enhanced DNA binding properties. Small 6:2256–2260

    Article  CAS  Google Scholar 

  10. Li Z, Zhang Y, Fullhart P, Mirkin CA (2004) Reversible and chemically programmable micelle assembly with DNA block-copolymer amphiphiles. Nano Lett 4:1055–1058

    Article  CAS  Google Scholar 

  11. Alemdaroglu FE, Ding K, Berger R, Herrmann A (2006) DNA-templated synthesis in three dimensions: introducing a micellar scaffold for organic reactions. Angew Chem Int Ed 45:4206–4210

    Article  CAS  Google Scholar 

  12. Jeong JH, Park TG (2001) Novel Polymer-DNA hybrid polymeric micelles composed of hydrophobic Poly(D, L-lactic-co-glycolic Acid) and hydrophilic oligonucleotides. Bioconjug Chem 12:917–923

    Article  CAS  Google Scholar 

  13. Chien M-P, Rush AM, Thompson MP, Gianneschi NC (2010) Programmable shape-shifting micelles. Angew Chem Int Ed 49:5076–5080

    Article  CAS  Google Scholar 

  14. Northwestern University. oligonucleotide properties calculator used to calculate extinction coefficient of DNA. http://www.basic.northwestern.edu/biotools/OligoCalc.html

  15. Sun S, Zeng H (2002) Size-controlled synthesis of magnetite nanoparticles. J Am Chem Soc 124:8204–8205

    Article  CAS  Google Scholar 

  16. Clegg RM, David M.J.L.a.J.E.D. (1992) Fluorescence resonance energy transfer and nucleic acids. Methods Enzymol, vol. 211, Academic, p 353–388

    Google Scholar 

  17. The information is available at the Glen Research website. Glen Research. http://www.glenresearch.com/

    Article  CAS  Google Scholar 

  18. Kuno M, Lee JK, Dabbousi BO, Mikulec FV, Bawendi MG (1997) The band edge luminescence of surface modified CdSe nanocrystallites: probing the luminescing state. J Chem Phys 106:9869–9882

    Article  CAS  Google Scholar 

  19. Zimmermann J, Kwak M, Musser AJ, Herrmann A (2011) Amphiphilic DNA block copolymers: nucleic acid-polymer hybrid materials for diagnostics and biomedicine. Methods Mol Biol 751:239–266

    Google Scholar 

Download references

Acknowledgments

This work was supported by the NSF Career Award (DMR-087646) and NSF-IGERT Fellowship (DGE-0221664).

Author information

Authors and Affiliations

  1. Department of Chemistry, University of Pennsylvania, Philadelphia, PA, USA

    Xi-Jun Chen, Robert J. Hickey & So-Jung Park

Authors
  1. Xi-Jun Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Robert J. Hickey
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. So-Jung Park
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Dipto di Ingegneria Meccanica, Università degli Studi di Brescia, Brescia, Italy

    Paolo Bergese

  2. Dept of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA

    Kimberly Hamad-Schifferli

Rights and permissions

Reprints and permissions

Copyright information

© 2013 Springer Science+Business Media New York

About this protocol

Cite this protocol

Chen, XJ., Hickey, R.J., Park, SJ. (2013). Preparation and Characterization of DNA Block Copolymer Assemblies Loaded with Nanoparticles. In: Bergese, P., Hamad-Schifferli, K. (eds) Nanomaterial Interfaces in Biology. Methods in Molecular Biology, vol 1025. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-462-3_16

Download citation

  • DOI: https://doi.org/10.1007/978-1-62703-462-3_16

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-461-6

  • Online ISBN: 978-1-62703-462-3

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation