Abstract
Cell shape and substrate rigidity play critical roles in regulating cell behaviors and fate. Controlling cell shape on elastic adhesive materials holds great promise for creating a physiologically relevant culture environment for basic and translational research and clinical applications. However, it has been technically challenging to create high-quality adhesive patterns on compliant substrates. We have developed an efficient and economical method to create precise micron-scaled adhesive patterns on the surface of a hydrogel (Rape et al., Biomaterials 32:2043–2051, 2011). This method will facilitate the research on traction force generation, cellular mechanotransduction, and tissue engineering, where precise controls of both materials rigidity and adhesive patterns are important.
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References
Tambe DT, Corey-Hardin C, Angelini TE et al (2011) Collective cell guidance by cooperative intercellular forces. Nat Mater 10:469–475
Janmey PA, Miller RT (2011) Mechanisms of mechanical signaling in development and disease. J Cell Sci 124:9–18
Chen CS, Mrksich M, Huang S et al (1997) Geometric control of cell life and death. Science 276:1425–1428
Singhvi R, Kumar A, Lopez G et al (1994) Engineering cell shape and function. Science 264:696–698
Dike L, Chen C, Mrksich M et al (1999) Geometric control of switching between growth, apoptosis, and differentiation during angiogenesis using micropatterned substrates. In Vitro Cell Dev Biol Anim 35: 441–448
McBeath R, Pirone DM, Nelson CM et al (2004) Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment. Dev Cell 6:483–495
Kilian KA, Bugarija B, Lahn BT et al (2010) Geometric cues for directing the differentiation of mesenchymal stem cells. Proc Natl Acad Sci 107:4872–4877
Wang H-B, Dembo M, Wang Y-L (2000) Substrate flexibility regulates growth and apoptosis of normal but not transformed cells. Am J Physiol Cell Physiol 279:C1345–C1350
Engler AJ, Sen S, Sweeney HL et al (2006) Matrix elasticity directs stem cell lineage specification. Cell 126:677–689
Pelham RJ, Wang Y-l (1997) Cell locomotion and focal adhesions are regulated by substrate flexibility. Proc Natl Acad Sci 94:13661–13665
Rape A, Guo W-H, Wang Y-L (2011) Responses of cells to adhesion-mediated signals: a universal mechanism. In: Wagoner JA, Harley BAC (eds) Mechanobiology of cell-cell and cell-matrix interactions. Springer, USA, pp 1–10
Wang Y-L, Pelham RJ Jr (1998) Preparation of a flexible, porous polyacrylamide substrate for mechanical studies of cultured cells. In: Richard BV (ed) Methods in enzymology. Academic, New York, pp 489–496
Damljanovic V, Lagerholm BC, Jacobson K (2005) Bulk and micropatterned conjugation of extracellular matrix proteins to characterized polyacrylamide substrates for cell mechanotransduction assays. Biotechniques 39:847–851
Guo W-h, Wang Y-I (2007) Retrograde fluxes of focal adhesion proteins in response to cell migration and mechanical signals. Mol Biol Cell 18:4519–4527
Wang N, Ostuni E, Whitesides GM et al (2002) Micropatterning tractional forces in living cells. Cell Motil Cytoskeleton 52:97–106
Vignaud T, Galland R, Tseng Q et al (2012) Reprogramming cell shape with laser nano-patterning. J Cell Sci 125:2134–2140
Rape AD, Guo W-h, Wang Y-l (2011) The regulation of traction force in relation to cell shape and focal adhesions. Biomaterials 32:2043–2051
Fischer B, Thomas KB, Dorner F (1998) Collagen covalently immobilized onto plastic surfaces simplifies measurement of von Willebrand factor-collagen binding activity. Ann Hematol 76:159–166
Li F, Li B, Wang Q-M et al (2008) Cell shape regulates collagen type I expression in human tendon fibroblasts. Cell Motil Cytoskeleton 65:332–341
Reinhart-King CA, Dembo M, Hammer DA (2003) Endothelial cell traction forces on RGD-derivatized polyacrylamide substrata. Langmuir 19:1573–1579
Guo W-H, Wang Y-l (2011) Micropatterning cell-substrate adhesions using linear polyacrylamide as the blocking agent. Cold Spring Harb Protoc 2011(3):prot5582
Acknowledgement
This work was supported by grant GM-32476 from the National Institutes of Health to Y.L.W.
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Zhang, J., Guo, Wh., Rape, A., Wang, Yl. (2013). Micropatterning Cell Adhesion on Polyacrylamide Hydrogels. In: Baudino, T. (eds) Cell-Cell Interactions. Methods in Molecular Biology, vol 1066. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-604-7_13
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DOI: https://doi.org/10.1007/978-1-62703-604-7_13
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