Abstract
The uncoating process of HIV-1 is a poorly understood process, so the development of a reliable assay to study uncoating is critical for moving the field forward. Here we describe an uncoating assay that currently represents the state-of-the-art biochemical procedure for monitoring uncoating and core stability during infection. This assay is based on the biochemical separation of soluble capsid protein from particulate capsid cores and provides information about the fate of the capsid during infection.
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Acknowledgments
This work was funded by an R01 AI087390 to F.D.-G, NIH grant RO1AI59159 to J.L., Swiss National Science Foundation grant 3100A0-128655 to J.L., and a K99/R00 Pathway to Independence Award to F.D.-G. from the National Institutes of Health 4R00MH086162-02.
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Yang, Y., Luban, J., Diaz-Griffero, F. (2014). The Fate of HIV-1 Capsid: A Biochemical Assay for HIV-1 Uncoating. In: Vicenzi, E., Poli, G. (eds) Human Retroviruses. Methods in Molecular Biology, vol 1087. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-670-2_3
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DOI: https://doi.org/10.1007/978-1-62703-670-2_3
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