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Isolated Neurohypophysial Terminals: Model for Depolarization–Secretion Coupling

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Exocytosis Methods

Part of the book series: Neuromethods ((NM,volume 83))

Abstract

Here we discuss the useful properties of a preparation of isolated neurosecretory nerve terminals obtained from mammalian neurohypophyses (posterior pituitaries). These nerve terminals release the two neuropeptides, oxytocin and vasopressin, which are easily assayed by radioimmunoassay and/or ELISA. Depolarization-induced exocytosis is dependent on the same parameters as those regulating release from the whole, intact neurohypophysis. The isolated nerve terminals can be identified and also imaged for intracellular Ca2+ and a functional synapse can be reconstituted using their purified neurosecretory granules. Furthermore, some nerve terminals are large enough to allow the use of patch-clamp techniques and the monitoring of exocytosis by capacitance and amperometric measurements. We also present evidence which show that the isolated neurohypophysial nerve terminals represent a powerful tool for studying the mechanisms underlying depolarization–secretion coupling (D-SC).

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Acknowledgements

Many thanks to the father of the “kikis,” Jean J. Nordmann, for developing and teaching us this wonderful preparation. We also wish to acknowledge seminal work in developing this preparation by Vincent Coccia, Govindan Dayanithi, Valerie DeCrescenzo, Alex Dopico, Thomas Knott, Joseph Lockhart, Karen Ocorr, Sonia Ortiz-Miranda, Mark Savage, Edward Stuenkel, Peter Thorn, Steven Treistman, Cristina Velazquez, Gang Wang, Xiaoming Wang, and Yong Yin. Supported by NIH grants: NS29470, DA10487 and NS063192 to J.R.L.

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Authors and Affiliations

  1. Department of Microbiology and Physiological Systems and Program Neuroscience, University of Massachusetts Medical School, Worcester, MA, USA

    José R. Lemos

  2. Department of Psychiatry, VA Boston Healthcare System, Harvard Medical School, Brockton, MA, USA

    James McNally

  3. Department Microbiology and Physiological Systems and Program Neuroscience, University of Massachusetts Medical School, Worcester, MA, USA

    Edward Custer

  4. Department of Microbiology and Physiological Systems and Program in Neuroscience, University of Massachusetts Medical School, Worcester, MA, USA

    Adolfo Cuadra

  5. Instituto de Neurobiología, UPR-MSC, San Juan, Puerto Rico

    Hector Marrero

  6. Department of Physiology and Developmental Biology, Brigham Young University, Provo, UT, USA

    Dixon Woodbury

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  1. José R. Lemos
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  2. James McNally
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  3. Edward Custer
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  4. Adolfo Cuadra
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  5. Hector Marrero
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  6. Dixon Woodbury
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Editors and Affiliations

  1. School of Biomedical Sciences, University of Queensland, St. Lucia, Queensland, Australia

    Peter Thorn

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Lemos, J.R., McNally, J., Custer, E., Cuadra, A., Marrero, H., Woodbury, D. (2014). Isolated Neurohypophysial Terminals: Model for Depolarization–Secretion Coupling. In: Thorn, P. (eds) Exocytosis Methods. Neuromethods, vol 83. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-676-4_10

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  • DOI: https://doi.org/10.1007/978-1-62703-676-4_10

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-675-7

  • Online ISBN: 978-1-62703-676-4

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