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Portable Two-Way Riboswitches: Design and Engineering

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Artificial Riboswitches

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1111))

Abstract

Riboswitches are RNA-based regulatory devices that mediate ligand-dependent control of gene expression. However, there has been limited success in rationally designing riboswitches. Moreover, most previous riboswitches are confined to a particular gene and only perform one-way regulation. Here, we describe a library screening strategy for efficient creation of ON riboswitches of lacI of Escherichia coli. An ON riboswitch of lacI is then integrated with the lac promoter, generating a hybrid device to achieve portable sequential OFF-and-ON gene regulation.

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Author information

Authors and Affiliations

  1. Department of Biochemistry, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong SAR, People’s Republic of China

    Ye Jin

  2. Department of Biochemistry and Shenzhen Institute of Research and Innovation, The University of Hong Kong, Pokfulam, Hong Kong

    Jian-Dong Huang

  3. The Center for Synthetic Biology Engineering Research, Shenzhen Institutes of Advanced Technology, Shenzhen, People’s Republic of China

    Jian-Dong Huang

Authors
  1. Ye Jin
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  2. Jian-Dong Huang
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Editor information

Editors and Affiliations

  1. Ehime University, Matsuyama, Ehime, Japan

    Atsushi Ogawa

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© 2014 Springer Science+Business Media New York

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Jin, Y., Huang, JD. (2014). Portable Two-Way Riboswitches: Design and Engineering. In: Ogawa, A. (eds) Artificial Riboswitches. Methods in Molecular Biology, vol 1111. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-755-6_7

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  • DOI: https://doi.org/10.1007/978-1-62703-755-6_7

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-754-9

  • Online ISBN: 978-1-62703-755-6

  • eBook Packages: Springer Protocols

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