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Purification of O2-Sensitive Metalloproteins

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Metalloproteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1122))

Abstract

The most dependable factor to perform successful biochemical experiments in an O2-free environment is the experience required to set up an efficient laboratory, to properly manipulate samples, to anticipate potential O2-related problems, and to maintain the complex laboratory setup operative. There is a long list of O2-related issues that may ruin your experiments. We provide here a guide to minimize these risks.

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Acknowledgments

This work was supported by ERC Starting Grant 205442 and by MICINN Grant BIO2009-12661.

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Authors and Affiliations

  1. Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, Campus de Montegancedo, Pozuelo de, Alarcón, Madrid, 28223, Spain

    Carlos Echavarri-Erasun, Simon Arragain & Luis M. Rubio

Authors
  1. Carlos Echavarri-Erasun
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  2. Simon Arragain
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  3. Luis M. Rubio
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Corresponding author

Correspondence to Luis M. Rubio .

Editor information

Editors and Affiliations

  1. Metalloproteins Unit, Institut de Biologie Structurale (IBS), Grenoble, France

    Juan C. Fontecilla-Camps

  2. Metalloproteins Unit, Institut de Biologie Structurale (IBS), Grenoble, France

    Yvain Nicolet

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Echavarri-Erasun, C., Arragain, S., Rubio, L.M. (2014). Purification of O2-Sensitive Metalloproteins. In: Fontecilla-Camps, J., Nicolet, Y. (eds) Metalloproteins. Methods in Molecular Biology, vol 1122. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-794-5_2

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  • DOI: https://doi.org/10.1007/978-1-62703-794-5_2

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-793-8

  • Online ISBN: 978-1-62703-794-5

  • eBook Packages: Springer Protocols

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