Abstract
Described herein are methods for the successful screening of monoclonal antibodies (mAbs) of the desired specificities via high-throughput (HTP) homogeneous assay and flow cytometry. We present a combination of screening techniques that allow the scientist to efficiently eliminate nontarget-specific antibody as soon as possible. This compilation of protocols will enable researchers with basic immunology skills to make decisions regarding the design of screening algorithms for the generation of mAbs. Although we have provided an informative overview of both HTP homogeneous assay and flow cytometry, it is imperative for the beginner to acquire fundamental knowledge on how both of these technologies work so as to use these screening strategies effectively.
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The views and opinions expressed herein are those of the authors only and do not necessarily represent the views and opinions of the Public Health Agency of Canada or the Government of Canada.
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Uribe-Benninghoff, A., Cabral, T., Chronopoulou, E., Berry, J.D., Corbett, C.R. (2014). Screening Hybridomas for Cell Surface Antigens by High-Throughput Homogeneous Assay and Flow Cytometry. In: Ossipow, V., Fischer, N. (eds) Monoclonal Antibodies. Methods in Molecular Biology, vol 1131. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-992-5_6
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DOI: https://doi.org/10.1007/978-1-62703-992-5_6
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-62703-992-5
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