Abstract
Eggplant (Solanum melongena L.) is an important vegetable crop of tropical and temperate regions of the world. Here we describe a procedure for eggplant plastid transformation, which involves preparation of explants, biolistic delivery of plastid transformation vector into green stem segments, selection procedure, and identification of the transplastomic plants. Shoot buds appear from cut ends of the stem explants following 5–6 weeks of spectinomycin selection after bombardment with the plastid transformation vector containing aadA gene as selectable marker. Transplastomic lines are obtained after the regenerated shoots are subjected to several rounds of spectinomycin selection over a period of 9 weeks. Homoplasmic transplastomic lines are further confirmed by spectinomycin and streptomycin double selection. The transplastomic technology development in this plant species will open up exciting possibilities for improving crop performance, metabolic engineering, and the use of plants as factories for producing biopharmaceuticals.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Kalloo G (1993) Eggplant (Solanum melongena). In: Kalloo G (ed) Genetic improvement of vegetable crops. Pergamon, Oxford, pp 587–604
Guri A, Sink KC (1988) Agrobacterium transformation of eggplant. J Plant Physiol 133:52–55
Filippone E, Lurquin PF (1989) Stable transformation of eggplant (Solanum melongena L.) by cocultivation of tissues with Agrobacterium tumefaciens carrying a binary plasmid vector. Plant Cell Rep 8:370–373
Rotino GL, Gleddie S (1990) Transformation of eggplant (Solanum melongena L.) using a binary Agrobacterium tumefaciens vector. Plant Cell Rep 9:26–29
Fari M, Nagy I, Csanyi M, Mityko J, Andrasfalvy A (1995) Agrobacterium mediated genetic transformation and plant regeneration via organogenesis and somatic embryogenesis from cotyledon leaves in eggplant (Solanum melongena L. cv. ‘Kecskemeti lila’). Plant Cell Rep 15:82–86
Kumar PA, Mandaokar A, Sreenivasu K, Chakrabarti SK, Bisaria S, Sharma SR, Kaur S, Sharma RP (1998) Insect-resistant transgenic brinjal plants. Mol Breed 4:33–37
Hanyu H, Murata A, Park EY, Okabe M, Billings S, Jelenkovic G, Pedersen H, Chin CK (1999) Stability of luciferase gene expression in a long term period in transgenic eggplant Solanum melongena. Plant Biotechnol 16:403–407
Franklin G, Sita GL (2003) Agrobacterium tumefaciens-mediated transformation of eggplant (Solanum melongena L.) using root explants. Plant Cell Rep 21:549–554
Singh A, Verma SS, Bansal KC (2010) Plastid transformation in eggplant (Solanum melongena L.). Transgenic Res 19:113–119
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497
Zoubenko OV, Allison LA, Svab Z, Maliga P (1994) Efficient targeting of foreign genes into the tobacco plastid genome. Nucleic Acids Res 22:3819–3824
Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325
Acknowledgment
KCB is thankful to Pal Maliga and Zora Svab for the initial training on plastid transformation under the auspices of Rockefeller Career Biotechnology Fellowship.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media New York
About this protocol
Cite this protocol
Bansal, K.C., Singh, A.K. (2014). Plastid Transformation in Eggplant. In: Maliga, P. (eds) Chloroplast Biotechnology. Methods in Molecular Biology, vol 1132. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-995-6_19
Download citation
DOI: https://doi.org/10.1007/978-1-62703-995-6_19
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-994-9
Online ISBN: 978-1-62703-995-6
eBook Packages: Springer Protocols