Abstract
Typically, Yersinia pestis contains three plasmids: pCD1, pMT1, and pPCP1. Although there have been many studies of the functions of these plasmids, little is known about how these plasmids act coordinately to promote the virulence of Y. pestis. Therefore, the construction of plasmid-less mutant strains is critical for further investigations of the relationships among these plasmids. In this chapter, I describe a method of curing plasmids from Y. pestis using plasmid incompatibility.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Perry RD, Fetherston JD (1997) Yersinia pestis – etiologic agent of plague. Clin Microbiol Rev 10(1):35–66
Prentice MB, Rahalison L (2007) Plague. Lancet 369(9568):1196–1207
Du Y, Rosqvist R, Forsberg A (2002) Role of fraction 1 antigen of Yersinia pestis in inhibition of phagocytosis. Infect Immun 70(3):1453–1460
Hinnebusch BJ, Fischer ER, Schwan TG (1998) Evaluation of the role of the Yersinia pestis plasminogen activator and other plasmid-encoded factors in temperature-dependent blockage of the flea. J Infect Dis 178(5):1406–1415
Zhou D, Tong Z, Song Y, Han Y, Pei D, Pang X, Zhai J, Li M, Cui B, Qi Z et al (2004) Genetics of metabolic variations between Yersinia pestis biovars and the proposal of a new biovar, microtus. J Bacteriol 186(15):5147–5152
Hutchens M, Luker GD (2007) Applications of bioluminescence imaging to the study of infectious diseases. Cell Microbiol 9(10):2315–2322
Ni B, Du Z, Guo Z, Zhang Y, Yang R (2008) Curing of four different plasmids in Yersinia pestis using plasmid incompatibility. Lett Appl Microbiol 47(4):235–240
Uraji M, Suzuki K, Yoshida K (2001) A new method for construction of Ti plasmid-less strains in Agrobacterium tumefaciens. Nucleic Acids Res Suppl 1:173–174
Uraji M, Suzuki K, Yoshida K (2002) A novel plasmid curing method using incompatibility of plant pathogenic Ti plasmids in Agrobacterium tumefaciens. Genes Genet Syst 77(1):1–9
Qian G, Wang Y, Qian D, Fan J, Hu B, Liu F (2012) Selection of available suicide vectors for gene mutagenesis using chiA (a chitinase encoding gene) as a new reporter and primary functional analysis of chiA in Lysobacter enzymogenes strain OH11. World J Microbiol Biotechnol 28(2):549–557
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Nature Singapore Pte Ltd.
About this protocol
Cite this protocol
Zhou, Y. (2018). Plasmid Curing in Yersinia pestis . In: Yang, R. (eds) Yersinia Pestis Protocols. Springer Protocols Handbooks. Springer, Singapore. https://doi.org/10.1007/978-981-10-7947-4_21
Download citation
DOI: https://doi.org/10.1007/978-981-10-7947-4_21
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-10-7946-7
Online ISBN: 978-981-10-7947-4
eBook Packages: Springer Protocols