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The In Vivo Isotopic Labeling of Proteins for Polyacrylamide Gel Electrophoresis

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Proteins

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 1))

Abstract

Autoradiography (see Chapter 17 and Vol. 2) offers a convenient, quick, wand cheap means of quantifying proteins separated by gel electrophoresis.

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References

  1. Bravo, R., and Celis, J. E. (1982) Up-dated catalogue of HeLa cell proteins. Percentages and characteristics of the major cell polypeptides labelled with a mixture of 16 14C-labelled amino acids. Clin. Chem. 28, 766–781.

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  2. Stanners, C. P., Adams, M. E., Harkins, J. L., and Pollard, J. W. (1979) Transformed cells have lost control of ribosome number through the growth cycle. J. Cell. Physiol. 100, 127–138.

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© 1984 Humana Press

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Pollard, J.W. (1984). The In Vivo Isotopic Labeling of Proteins for Polyacrylamide Gel Electrophoresis. In: Walker, J.M. (eds) Proteins. Methods in Molecular Biology™, vol 1. Humana Press. https://doi.org/10.1385/0-89603-062-8:75

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  • DOI: https://doi.org/10.1385/0-89603-062-8:75

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-062-6

  • Online ISBN: 978-1-59259-488-7

  • eBook Packages: Springer Protocols

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