Abstract
Since the identification of HIV (human immunodeficiency virus) as the retrovirus responsible for AIDS (acquired immunodeficiency syndrome), huge efforts have been made to identify compounds possessing antiviral activity. Many assay systems are available to measure various stages of the viral lifecycle. These are very convenient for assessing the antiviral activity of compounds. The assay systems often use T-cell lines expressing CD4 on their surface, since this molecule is an essential component of the receptor for the virus (1). The basic principle when testing for anti-HIV compounds involves the interaction of virus with test material under suitable culture conditions and the subsequent measurement of a parameter reflecting the amount of virus present and, thus, the degree of viral replication.
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References
Dalgleish, A. C., Beverley, P C. L., Clapham, P. R., Crawford, D. R., Greaves, M. F., and Weiss, R. A. (1984) The CD4 (TL) antigen is an essential component of the receptor for the AIDS retrovirus. Nature 312, 763–767.
Pauweh, R., Balzarini, J., Baba, M., Snoeck, R., Schols, D., Herdewijn, P., Resmyter, J, and De Clercq, E. (1988) Rapid and automated tetrarolium-based colorimetric assay for detection of anti-HIV compounds. J. Virol. Methods 20, 309–321.
Goff, S., Traktman, P., and Baltimore, D. (1981) Isolation properties of Moloney murine leukemia virus mutants: Use of a rapid assay for release of virion reverse transcriptase. J. Virol. 38, 239–248.
Boyd, M. T., Maclean, N., and Oscier, D. G. (1989) Detection of retrovirus in pauents with myeloproliferative disease. Lancet i, 814–817.
Spence, R. P., Jarvill, W. M., Ferns, R. C., Tedder, R. S., and Parker, D. (1989) The cloning and expression in E. Coli of sequences coding for p24, the core protein of human immunodeficiency virus and the use of the recombinant protein in characterizing a panel of monoclonal antibodies against the viral p24 protein. J. Gen. Virol. 70, 2843–2851.
Ferns, R. B., Partridge, J. C, Spence, R. P., Hunt, N., and Tedder, R. S. (1989) Epitope location of 13 anti-gag HIV-1 monoclonal antibodies and their cross reactivity with HIV-2. AIDS 3, 829–834.
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© 1991 The Humana Press Inc., Clifton, NJ
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Patience, C., Moore, J., Boyd, M. (1991). Assessment of Compounds for Anti-HIV Activity. In: Collins, M.K.L. (eds) Practical Molecular Virology. Methods in Molecular Biology, vol 8. Humana Press. https://doi.org/10.1385/0-89603-191-8:131
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DOI: https://doi.org/10.1385/0-89603-191-8:131
Publisher Name: Humana Press
Print ISBN: 978-0-89603-191-3
Online ISBN: 978-1-59259-495-5
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