Abstract
The isolation and subsequent study of hepatocytes in in vitro conditions was first transformed by Berry and Friend (1), who developed a collagenase liver perfusion method, allowing the isolation of large numbers of cells with high viability.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Berry, M. N. and Friend, D. S. (1969) High-yield preparation of isolated rat liver parenchymal cells. J. Cell. Biol. 43, 506–520.
Seglen, P. O. (1976) Preparation of isolated rat liver cells, in Methods in Cell Biology, vol. 13 (Prescott, D. M., ed.), Academic, New York, pp. 29–83.
Davies, R., Cain, K., Edwards, R. E. Snowden, R. T., Legg, R. F., and Neal, G. E. (1990) The preparation of highly enriched fractions of binucleated rat hepatocytes by centrifugal elutriation and flow cytometry. Anal. Biochem. 190, 266–270.
Meredith, M. J. (1988) Rat hepatocytes prepared without collagenase: Prolonged retention of differentiated characteristics in culture. Cell Biol. Toxicol. 4, 405–425.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1992 The Humana Press, Inc., Totowa, NJ
About this protocol
Cite this protocol
Davies, R. (1992). The Isolation of Rat Hepatocytes for Flow Cytometry. In: Manson, M.M. (eds) Immunochemical Protocols. Methods in Molecular Biology, vol 10. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-204-3:369
Download citation
DOI: https://doi.org/10.1385/0-89603-204-3:369
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-204-0
Online ISBN: 978-1-59259-497-9
eBook Packages: Springer Protocols