Abstract
Central to the process of making transgenic mice is the physical introduction of cloned DNA fragments into fertilized one-cell mouse eggs. First described 10 years ago by a number of investigators (1–5), microinjection remains the most popular and successful of the methods currently available for generating transgenic animals. Microinjection continues to be the method of choice, because the advantages of speed and reliability far outweigh the demands placed on the investigator for precise technical skill and expensive equipment.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Wagner, E. F., Stewart, T. A., and Mintz, B. (1981) The human beta-globin gene and a functional thymidine kinase gene in developing mice. Proc. Nutl. Acad. Sci. USA 78, 5016–5050.
Wagner, T. E., Hoppe, P. C., Jollick, J. D., Scholl, D. R., Hodinka, R. L, and Gault, J. B. (1981) Microinjection of rabbit beta-globin gene into zygotes and subsequent expression in adult mice and their offspring. Proc. Natl. Acad. Sci. USA 78, 6376–6380.
Brinster, R. L., Chen, H. Y., Trumbauer, M., Senear, A. W., Warren, R., and Palmiter R. D. (1981) Somatic expression of herpes thymidine kinase in mice following injection of fusion genes into eggs. Cell 27, 223–231
Constantini, F. and Lacy, E. (1981) Introduction of a rabbit beta-globin gene into the mouse germ line. Nature 294, 92–94.
Gordon, J. W. and Ruddle, F. H. (1981) Integration and stable transmission of genes injected into mouse pronuclei. Science 214, 1244–1246.
Hogan, B. L. M., Constantini, F., and Lacy, E. (1986) Manipulating the Mouse Embryo—A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Brinster, R. L, Chen, H. Y., Trumbuaer, M. E., Yagle, M. K., and palmiter, R. D. (1985) Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs. Proc. Natl. Acad. Sci. USA 82, 4438–4442.
Grosschedl, R., Weaver, D., Baltimore, D., and Constantini, F (1984) Introduction of μ immunoglobulin gene into the mouse germline: specific expression in lymphoid cells and synthesis of functional antibody. Cell 38, 647–658.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1993 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Murphy, D. (1993). Microinjection of Cloned DNA Fragments into Fertilized One-Cell Mouse Eggs. In: Murphy, D., Carter, D.A. (eds) Transgenesis Techniques. Methods in Molecular Biology™, vol 18. Humana Press. https://doi.org/10.1385/0-89603-245-0:151
Download citation
DOI: https://doi.org/10.1385/0-89603-245-0:151
Publisher Name: Humana Press
Print ISBN: 978-0-89603-245-3
Online ISBN: 978-1-59259-505-1
eBook Packages: Springer Protocols