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Expression and Secretion of a Soluble Form of Myelin-Associated Glycoprotein (MAG)

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Baculovirus Expression Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 39))

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Abstract

Myelin-associated glycoprotein (MAG) was first identified as the major glycoprotein in the central nervous system (CNS) through 3Hfucose-labeling experiments (1). It is expressed on the surface of glial cells of both the CNS and peripheral nervous system (PNS). During development, it is localized throughout the wraps of loose myelin; after compaction, it is restricted to Schmidt-Lanterman incisures, paranodal loops, outer mesaxon, and in particular to the glial-axon interface (2, 3). The protein is thought to play a role in maintaining the periaxonal space. MAG has also been postulated to be involved in glial-neuron adhesion during myelination. This is based on the finding that anti-MAG antibodies inhibit oligodendrocyte-neuron adhesion by 25% (4), and that MAG liposomes bind a variety of neuronal cells (5). Observations from in vitro myelination cultures also support a role for MAG in Schwann cell adhesion, migration, and elongation along neurites (6, 7).

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Author information

Authors and Affiliations

  1. Centre for Research in Neuroscience, McGill University, Montreal General Hospital Research Institute, Montreal, Quebec, Canada

    John Attia, Sanjoy Gupta & Robert J. Dunn

Authors
  1. John Attia
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  2. Sanjoy Gupta
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  3. Robert J. Dunn
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Editor information

Editors and Affiliations

  1. Amgen and Ontario Cancer Institutes, Toronto, Canada

    Christopher D. Richardson

  2. Department of Medical Biophysics, University of Toronto, Canada

    Christopher D. Richardson

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© 1995 Humana Press Inc, Totowa, NJ

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Attia, J., Gupta, S., Dunn, R.J. (1995). Expression and Secretion of a Soluble Form of Myelin-Associated Glycoprotein (MAG). In: Richardson, C.D. (eds) Baculovirus Expression Protocols. Methods in Molecular Biology, vol 39. Humana Press. https://doi.org/10.1385/0-89603-272-8:363

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  • DOI: https://doi.org/10.1385/0-89603-272-8:363

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-272-9

  • Online ISBN: 978-1-59259-526-6

  • eBook Packages: Springer Protocols

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