Abstract
As solid-phase peptide synthesis techniques improved and the rate at which a peptide could be synthesized increased, purification was identified as the new bottleneck in the production of high-quality peptides. Purification took a giant leap forward with the introduction of reversed-phase high-performance liquid chromatography (RP-HPLC; see Chapter 3) to the synthetic laboratory. In fact, HPLC technology has been instrumental in the purification and characterization of most biologically active peptides and proteins (1).
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© 1994 Humana Press Inc., Totowa, NJ
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Byrnes, M.E. (1994). Reversed-Phase High-Performance Liquid Chromatography. In: Dunn, B.M., Pennington, M.W. (eds) Peptide Analysis Protocols. Methods in Molecular Biology, vol 36. Humana Press. https://doi.org/10.1385/0-89603-274-4:37
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DOI: https://doi.org/10.1385/0-89603-274-4:37
Publisher Name: Humana Press
Print ISBN: 978-0-89603-274-3
Online ISBN: 978-1-59259-523-5
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