Abstract
The group of De Duve first described the isolation of peroxisomes from rat liver tissue. These organelles can be separated from other sub-cellular organelles, such as lysosomes and mitochondria, because of their relatively high equilibrium density in sucrose (approx 1.24 g/cm3). The isolation of peroxisomes from the yeast Saccharomyces cerevisiae, however, has been hampered by the fact that under standard growth conditions peroxisomes are present in low numbers. Also, the lability of peroxisomes in general has complicated their purification.
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© 1996 Humana Press Inc., Totowa, NJ
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Distel, B., van der Leij, I., Kos, W. (1996). Peroxisome Isolation. In: Evans, I.H. (eds) Yeast Protocols. Methods in Molecular Biology™, vol 53. Humana Press. https://doi.org/10.1385/0-89603-319-8:133
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DOI: https://doi.org/10.1385/0-89603-319-8:133
Publisher Name: Humana Press
Print ISBN: 978-0-89603-319-1
Online ISBN: 978-1-59259-540-2
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