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Protein Sequencer Maintenance and Troubleshooting

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Protein Sequencing Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 64))

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Abstract

Modern automated sequencers, both gas-phase and pulsed-liquid forms, are normally efficient and reliable; this chapter is intended for use when they are not. The author’s own experiences are almost wholly concerned with ABI (Applied Biosystems) instruments; however, the principles described will apply to most other manufacturers’ sequencers. This chapter will address the various functions individually, in an attempt to identify typical faults and remedies as outlined in Table 1 and Notes 115.

Table 1 A Trouble-Shooting Guide for Protein Sequencers

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References

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© 1997 Humana Press Inc.

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Dunbar, B. (1997). Protein Sequencer Maintenance and Troubleshooting. In: Smith, B.J. (eds) Protein Sequencing Protocols. Methods in Molecular Biology™, vol 64. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-353-8:217

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  • DOI: https://doi.org/10.1385/0-89603-353-8:217

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-353-5

  • Online ISBN: 978-1-59259-550-1

  • eBook Packages: Springer Protocols

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