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Preparation of Homogeneous Ribozyme RNA for Crystallization

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Ribozyme Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 74))

Abstract

The development of in vitro transcription methods has facilitated research in RNA structural biology in recent years. There is now increasing interest in determining atomic resolution structures of RNA by X-ray crystallography. The first step in this process is the production of a homogeneous sample of the RNA molecule of interest for crystallization trials.

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© 1997 Humana Press Inc.

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Doudna, J.A. (1997). Preparation of Homogeneous Ribozyme RNA for Crystallization. In: Turner, P.C. (eds) Ribozyme Protocols. Methods in Molecular Biology™, vol 74. Humana Press. https://doi.org/10.1385/0-89603-389-9:365

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  • DOI: https://doi.org/10.1385/0-89603-389-9:365

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-389-4

  • Online ISBN: 978-1-59259-560-0

  • eBook Packages: Springer Protocols

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