Abstract
M13 bacteriophage has a single-stranded DNA (ssDNA) genome, and has proven an extremely useful vector from which to derive single-stranded templates for sequencing and site-directed mutagenesis. During infection of its host cell, the phage DNA replicates as a double-stranded intermediate from which the ssDNA containing phage particles are produced. Infected cells do not lyse, but instead phage particles are continuously released. Cells infected with M13 phage, however, have a longer replication cycle, which means that as the infection proceeds, the areas of slower-growing cells can be visualized as turbid plaques on the lawn of unaffected E. coli (1). Recombinant M13 phage can be cloned from well-separated plaques and used as a source of SSDNA.
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References
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© 1996 Humana Press Inc., Totowa, NJ
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Tomley, F.M. (1996). M13 Phage Growth and Single-Stranded DNA Preparation. In: Harwood, A.J. (eds) Basic DNA and RNA Protocols. Methods in Molecular Biology™, vol 58. Humana Press. https://doi.org/10.1385/0-89603-402-X:359
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DOI: https://doi.org/10.1385/0-89603-402-X:359
Publisher Name: Humana Press
Print ISBN: 978-0-89603-402-0
Online ISBN: 978-1-59259-251-7
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