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Proliferation of Murine Myoblasts as Measured by Bromodeoxyuridine Incorporation

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Basic Cell Culture Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 75))

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Abstract

Growth factors or cytokines have been implicated in a wide variety of physiological and pathological processes. It has been shown that growth factors bind to specific receptors, and generate multiple signals that interact synergistically to initiate a proliferative response (1). Synergistic effects are of crucial importance in assessing the growth-promoting activity of any agent in low serum or serum free, chemically defined conditions.

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References

  1. Rozengurt, E. (1991) Measurement of the proliferative effects of cytokines, in Cytokines: A Practical Approach (Balkwill, F. R., ed.), IRL Press, Oxford, UK, pp. 253–260.

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  2. Freshney, I. R. (1987) Quantitation and experimental design, in Culture of Animal Cells: A Manual of Basic Technique, Chapter 18, LISS, New York, pp. 227–244.

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  3. Kaufman, S. J. and Foster, R. F. (1988) Replicating myoblasts express a muscle-specific phenotype. Proc. Natl. Acad. Sci. USA 85, 9606–9610.

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© 1997 Humana Press Inc., Totowa, NJ

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Shah, B., Hyde-Dunn, J., Jones, G.E. (1997). Proliferation of Murine Myoblasts as Measured by Bromodeoxyuridine Incorporation. In: Pollard, J.W., Walker, J.M. (eds) Basic Cell Culture Protocols. Methods in Molecular Biology™, vol 75. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-441-0:349

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  • DOI: https://doi.org/10.1385/0-89603-441-0:349

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-441-9

  • Online ISBN: 978-1-59259-561-7

  • eBook Packages: Springer Protocols

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