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Nosocomial Infections Caused by Staphylococci

  • Protocol
Molecular Bacteriology

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 15))

Abstract

Staphylococcus aureus and coagulase-negative species of staphylococci (CNS), particularly S epidermidis, are among the most frequently isolated bacteria from patients with nosocomial infection. Conventional methods used in the clinical microbiology laboratory for identification, susceptibility testing, and epidemiologic typing of staphylococci have several limitations Identification to species level is often limited to S. aureus, based on ability to produce coagulase, thermostable nuclease, and clumping factor. However, several newly-described species of staphylococci may express some of these characters, whereas atypical strains of S. aureus do not, leading to misidentification (1,2). Furthermore, among the 17 species of staphylococci currently recognized to be indigenous to humans, the potential of S. lugdunensis to cause serious infections is being increasingly recognized (1,2) Therefore, further speciation of CNS is clinically relevant. Distinction of true CNS infection from specimen contamination by skin flora can be addressed by demonstrating the repeated isolation of the same clone from multiple specimens, e g., blood cultures (1). Currently available, rapid biochemical test systems enable identification of staphylococci to species level with 60–90% accuracy (1). Antimicrobial susceptibility pattern, the only routinely available strain marker, is not reliable for clonal delmeation because of phenotypic variation among clonally derived isolates (1).

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References

  1. Kloos, W. E and Bannerman, T. L (1994) Update on clinical significance of coagulase-negative Staphylococci Clin Microbiol Rev 7, 117–140

    PubMed  CAS  Google Scholar 

  2. Vandenesch, F., Eykyn, S J.,and Etienne, J (1995) Infections caused by newly-described species of coagulase-negative staphylococci. Rev Med Microbiol 6, 94–100

    Google Scholar 

  3. Hartman, B. J. and Tomasz, A. (1984) Low-affinity penicillin-binding protein associated with μ-lactamase resistance in Staphylococcus aureus J. Bacteriol 158, 513–516

    PubMed  CAS  Google Scholar 

  4. Chambers, H. F (1988) Methicillin-resistant staphylococci. Clin Microbiol Rev 1, 173–186

    PubMed  CAS  Google Scholar 

  5. Bannerman, T. L, Hancock, G. A, Tenover, F C., and Miller, J. M (1995)Pulsed-field gel electrophoresis as a replacement for bacteriophage typing of Staphylococcus aureus J Clin Microbiol 33,551–555

    PubMed  CAS  Google Scholar 

  6. Thomson-Carter, F. M, Carter, P E, and Pennington, T H. (1989) Differentiation of staphylococcal species and strains by ribosomal RNA gene restriction patterns J Gen Mlcrobiol 135, 2093–2097.

    CAS  Google Scholar 

  7. Bialkowska-Hobrzanska, H, Harry, V, Jaskot, D., and Hammerberg, o (1990) Typing of coagulase-negative staphylococci by southern hybridization of chromosomal DNA fingerprints using a ribosomal RNA probe Eur J Clan Mlcrobiol Infect Dzs. 9, 588–594

    Article  CAS  Google Scholar 

  8. De Buyser M L., Morvan A, Grimont F, and El Solh N (1989) Characterization of Staphylococcus species by ribosomal RNA gene restriction patterns J Gen Microbiol 135, 989–999

    PubMed  Google Scholar 

  9. De Buyser, M L, Morvan, A, Aubert, S, Dilasser, F, and El Solh, N (1992) Evaluation of a ribosomal RNA gene probe for the identification of species and subspecies within the genus Staphylococcus J Gen Microbiol 138, 88–899

    Google Scholar 

  10. Chesneau, 0, Aubert, S., Morvan, A, Guesdon, J. L., and El Solh, N. (1992) Usefulness of the ID32 Staph System and a method based on rRNA gene restriction site polymorphism analysis for species and subspecies identification of staphylococcal clinical isolates J Clin Microbiol 30, 234&–2352

    Google Scholar 

  11. Chesneau, 0, Morvan, A, Grimont, F, Labischinski, H, and El Solh, N (1993) Staphylococcus Pasteuri sp. nov., isolated from human, animal, and food specimens. Int J Syst Bacteriol 43, 237–244

    Article  PubMed  CAS  Google Scholar 

  12. Freney, J, Meugnier, H., Bes, M., and Fleurette, J. (1993) Identification of Staphylococcus aureus using a DNA probe AccuprobeR Ann Biol Clin 51, 637–639

    CAS  Google Scholar 

  13. Davis, T E and Fuller, D D. (1991) Direct identification of bacterial isolates in blood cultures by using a DNA probe. J Clin Microbiol 29, 2193–2196

    PubMed  CAS  Google Scholar 

  14. Chesneau, O., Alhgnet, J, and El Solh, N. (1993) Thermonuclease gene as a target nucleotide sequence for specific recognition of Staphylococcus aureus Mol Cell Probes 7, 301–310.

    Article  PubMed  CAS  Google Scholar 

  15. Goh, S H., Potter, S., Wood, J. 0, Hemmingsen, S. M., Reynolds, R. P, and Chow, A. W. (1996) HSP60 gene sequences as universal targets for microbial species identification studies with coagulase-negative staphylococci. J Clin Microbiol 34, 818–823

    PubMed  CAS  Google Scholar 

  16. Ligozzi, M, Rossolini, G. M, Tonin, E. A., and Fontana, R. (1991) Nonradioactive DNA probe for detection of gene for methicillin resistance in Staphylococcus aureus Antimicrob Agents Chemother 35, 575–578.

    CAS  Google Scholar 

  17. .Predari, S. C, Ligozzi, M., and Fontana, R (1991) Genotypic identification of methicillin-resistant coagulase-negative staphylococci by polymerase chain reaction. Antimicrob Agents Chemother 35, 2568–2573

    PubMed  CAS  Google Scholar 

  18. .Archer, G L. and Pennell, E (1990) Detection of methicillin resistance in staphylococci by using a DNA probe Antimicrob Agents Chemother 34, 1720–1724

    PubMed  CAS  Google Scholar 

  19. de Lancastre, H, SaFigueiredo, A, Urban, C., Rahal, J., and Tomasz, A (1991) Multiple mechanisms of methicillin resistance and improved methods for detection in clinical isolates of Staphylococcus aureus Antimicrobial Agents Chemother 35, 632–639

    Google Scholar 

  20. .Shimaoka, M, Yoh, M., Segawa, A, Takarada, Y, Yamamoto, K, and Honda, T. (1994) Development of enzyme-labeled oligonucleotide probe for detection of mecA gene in methicillin-reststant Staphylococcus aureus J Clin Microbiol 32, 1866–1869

    PubMed  CAS  Google Scholar 

  21. .Richard, P, Meyran, M., Carpentier, E, Thabaut, A., and Drugeon, H. B. (1994) Comparison of phenotypic methods and DNA hybridization for detection of methicillin-resistant Staphylococcus aureus J Clin Microbiol 32, 613–617

    PubMed  CAS  Google Scholar 

  22. Kolbert, C P, Connolly, J E, Lee, M J, and Persing, D H (1995) Detection of the staphylococcal mecA gene by chemiluminescent DNA hybridization J CIin Microbiol 33, 2179–2182

    CAS  Google Scholar 

  23. Unal, S., Hoskins, J, Flokowitsch, J E, Wu, C Y E, Preston, D A, and Skatrud, P. L (1992) Detection of methxillin-resistant Staphylococci by using the polymerase chain reaction J Clin Microbiol 30,1685–1691

    PubMed  CAS  Google Scholar 

  24. .Kizaki, M, Kobayashi, Y, and Ikeda, Y. (1994) Rapid and sensitive detection of the femA gene in staphylococci by enzymatic detection of polymerase chain reaction (ED-PCR) comparison with standard PCR analysis.J Hosp Infect 28, 287–295

    Article  PubMed  CAS  Google Scholar 

  25. Brakstad, 0 D, Aasbakk, K., and Maeland, J. A (1992) Detection of Staphylococcus aureus by Polymerase Chain Reaction amplification of the nucgene J Clin Microbiol 30, 1654–1660

    PubMed  CAS  Google Scholar 

  26. Welsh, J and McClelland, M. (1992) PCR-amplified length polymorphisms in tRNA intergenic spacers for categorizing staphylococci. Mol Microbiol 6, 1673–1680.

    Article  PubMed  CAS  Google Scholar 

  27. Jensen, M A., Webster, J A., and Straus, N (1993) Rapid identification of bacterra on the basis of Polymerase Chain Reaction-amplified ribosomal DNA spacer polymorphisms Appl Environ Microbiol 59, 945–952.

    PubMed  CAS  Google Scholar 

  28. Maes, N, De Gheldere, Y, DeRyck, R, Vancechoutte, M, Meugnier, M, Etienne, J, and Struelens, M. J. (1997) Rapid and accurate identification of Staphylococcus species by tRNA intergenic sporer length polymorphism analysis J Clin Microbiol 35, 2477–2481

    PubMed  CAS  Google Scholar 

  29. Murakami, K, Minamide, W, Wada, K, Nakamura, E, Teraoka, H, and Watanabe, S. (1991) Identification of methicillin-resistant strains of staphylococci by Polymerase Chain Reaction J. Clin Microbiol 29, 2240–2244

    PubMed  CAS  Google Scholar 

  30. Tokue, Y, Shoji, S, Satoh, K., Watanabe, A, and Motomiya, M. (1992). Comparison of a Polymerase Chain Reaction assay and a conventional microbiologic method for detection of methicillin-resistant Staphylococcus aureus Antimicrob Agents Chemother 36, 6–8.

    CAS  Google Scholar 

  31. Ubukata, K., Nakagami, S, Nitta, A, Yamane, A., Kawakami, S, Sugmra, M, and Konno, M. (1992) Rapid detection of the mecA gene in methicillin-resistant staphylococci by enzymatic detection of Polymerase Chain Reaction products J Clin Microbiol 30, 1728–1733.

    PubMed  CAS  Google Scholar 

  32. Unal, S., Werner, K., DeGirolami, P, Barsanti, F., and Eliopoulos, G. (1994) Comparison of tests for detection of methicillin-resistant itStaphylococcus aureus in a clinical microbiology laboratory) Antimicrobial Agents Chemother [bd38], 345–347

    Google Scholar 

  33. Zambardi, G, Reverdy, M E., Bland, S., Bes, M., Freney, J, and Fleurette, J. (1994) Laboratory diagnosis of oxacillin resistance in Staphylococcus aureus by a multiplex Polymerase Chain Reaction assay. Diagn Microbiol Infect DES 19, 25–31

    Article  CAS  Google Scholar 

  34. Geha, D. J, Uhl, J R, Gustaferro, C A, and Persing, D H (1994) Multiplex PCR for identification of methicillin-resistant staphylococci in the clinical laboratory J CIin Microbiol 32, 1768–1772

    CAS  Google Scholar 

  35. Brakstad, O. G., Maeland, J. A., and Tveten, Y. (1993) Multiplex polymerase chain reaction for detection of genes for Staphylococcus aureus thermonuclease and methicillin resistance and correlation with oxacillin resistance APMIS 101, 681–688.

    Article  PubMed  CAS  Google Scholar 

  36. Vannuffel, P., Gigi, J., Ezzedine, H, Vandercam, B, Delmee, M, Wauters, G., and Gala, J L (1995) Specific detection of methicillin-resistant Staphylococcus species by multiplex PCR. J. Clin Microbiol 33, 2864–2867.

    PubMed  CAS  Google Scholar 

  37. Arbeit, R D (1995) Laboratory procedures for the epidemiologic analysis of microorganisms, in Manual of Clinical Microbiology, (Murray, P. R, Barton, E J, Pfeller, M. A., Tenover, F. C, and Yolken, R. H.), American Society for Microbiology, Washington, DC,. 190–208.

    Google Scholar 

  38. Struelens, M J and the European Strudy Group on Epidemiological Markers (1996) Consensus guidelines for appropriate use and evaluation of microbial epidemiologic typing systems Clin Microbial Infect 2, 2–11

    Article  Google Scholar 

  39. Struelens, M. J (1996) Laboratory methods in the investigation of outbreaks of hospital-acquired infection, inSurveillance of Nosocomial Infections, (Emmerson, A M and Ayhffe, G. A J., eds.), Ballhere’ s Clin Infect Dis 3, 267–288

    Google Scholar 

  40. Tenover, F C, Arbert, R D., Goering, R V, Mickelsen, P A., Murray, B E., Persing, D H., and Swaminathan, B. (1995) Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis. criteria for bacterial stram typing.J Clin. Microbiol 33, 2233–2239

    PubMed  CAS  Google Scholar 

  41. Hunter, P. R. (1990) Reproducibility and indices of discriminatory power of microbial typing methods. J Clin Microbiol 28, 1903–1905

    PubMed  CAS  Google Scholar 

  42. Parisi, J. T. and Hecht, D W. (1980) Plasmid profiles in epidemiologic studies of infections by Staphylococcus epidermidis J Infect Dis 141, 637–643

    Article  CAS  Google Scholar 

  43. Archer, G L, Vishniavsky, N., and Stiver, H G. (1982) Plasmid pattern analysis of Staphylococcus epidermidis isolates from patients with prosthetic valve endocarditis Infect immun 35, 627–632

    PubMed  CAS  Google Scholar 

  44. Archer, G. L, Karchmer, A. W, Vishniavsky, N, and Johnston, L (1984) Plasmid-pattern analysis for the differentiation of infecting from noninfecting Staphylococcus epidermidis J Infect. Dis 149, 913–920.

    Article  CAS  Google Scholar 

  45. Mickelsen, P. A., Plorde, J. J., Gordon, K. P, Hargiss, C., McClure, J, Fritz, D, Schoenknecht, F. D., Condie, F, Tenover, F C., and Tompkins, L S (1985) Instability of antibiotic resistance in a strain of Staphylococcus epidermidis isolated from an outbreak of prosthetic valve endocarditis.J Infect. Dis.152, 50–58

    Article  PubMed  CAS  Google Scholar 

  46. Valentine, C R., Yandle, S H., Marsik, F J, Ebright, J. R, and Dawson, M. S. (1988) Evaluation of the variety of plasmid profiles in S epidermidis isolates from hospital patients and staff Infect Control Hosp Epidemiol 9, 441–446

    Article  PubMed  CAS  Google Scholar 

  47. Etienne, J, Brun, Y, El Solh, N, Delorme, V, Mouren, C., Bes, M, and Fleurette, J (1988) Characterlization of clinically significant isolates ofStaphylococcus epidermidis from patients with endocarditis J Clin Microbiol 26, 613–617

    PubMed  CAS  Google Scholar 

  48. Ludlam, H A, Noble, W C, Marples, R R, and Phillips I (1989) The evaluation of a typing scheme for coagulase-negative staphylococci suitable for epidemiological studies.J Med Microbiol 30, 161–165.

    Article  PubMed  CAS  Google Scholar 

  49. Thore, M, Kuhn, I, Lofdahl, S., and Burman, L G (1990) Drug-resistant coagulase-negative skin staphylococci Epidemiol Infect 105, 95–105

    Article  PubMed  CAS  Google Scholar 

  50. Etienne, J, Bes, F, Renaud,M, Brun, Y, Greenland, T B, Freney, J, and Fleurette, J (1990) Instability of characteristics among coagulase-negative staphylococci causing endocarditis J Med Microbiol 32, 115–122

    Article  PubMed  CAS  Google Scholar 

  51. Hartstein, A. I, Morthland, V. H, and Rashad, A L (1991) Reproducibility of Staphylococcus epidermidis plasmid profiles. Diagn Microbiol Infect Dis 14, 275–280

    Article  PubMed  CAS  Google Scholar 

  52. Wilton, J., Jung, K., Vedin, I, Aronsson, B, and Flock, J I (1992) Comparative evaluation of a new molecular method for typing Staphylococcus epidermidis Eur J Clin Microbiol Infect Dis 11, 515–521

    Article  PubMed  CAS  Google Scholar 

  53. Dryden, M S., Talsama, H. G, Martin, S., Cunningham, M., Richardson, J F., Cookson, B, Marples, R R, and Phillips, I (1992) Evaluation of methods for typing coagulase-negative staphylococci J Med Microbiol.37, 109–117

    Article  PubMed  CAS  Google Scholar 

  54. Herwaldt, L A, Hollis, R J., Boyken, L D, and Pfaller, M. A (1992) Molecular epidemlology of coagulase-negative staphylococci isolated from unmunocompromised patients Infect Control and Hosp Epidemiol 13, 86–92.

    Article  CAS  Google Scholar 

  55. Loncle, V, Casetta, A., BUU-HOI, A., and El Solh, N. (1993) Analysis ofpnstmamycm-resistant Staphylococcus eprdermzdzs isolates responsible foran outbreak in a Parlslan hospital Antzmzcrob Agents Chemother 37, 2159–2165

    CAS  Google Scholar 

  56. Renaud, F., Etlenne, J, Bertrand, A, Brun, Y, Greenland, T B, Freney, J, and Fleurette, J (1991) Molecular epidemiology of Staphylococcus haemolyticus strains isolated in an Albanian hospital J Clin Microbiol.29, 1493–1497

    PubMed  CAS  Google Scholar 

  57. Etlenne, J., Poltevin-Later, F., Renaud, F, and Fleurette, J (1990) Plasmid profiles and genomic DNA restriction endonuclease patterns of 30 independent Staphylococcus lugdunensis strams FEMS Microbiol Lett 67, 93–98

    Article  Google Scholar 

  58. Bigelow, N, Ng, L-K., Robson, H G, and Dillon, J R (1989) Strategies for molecular charactersation of methicillin-and gentamicin-resistant staphylococcus aureus in a Canadian nosocomial outbreak. J Med Microbiol 30, 51–58

    Article  PubMed  CAS  Google Scholar 

  59. Coia, J. E, Hussain-Noor, I., and Platt, D. J (1988) Plasmid profiles and restriction enzyme fragmentation patterns of plasmids of methicillin-sensitive and methicillin-resistant isolates of Staphylococcus aureus from hospital and the community J Med Microbiol 27, 271–276.

    Article  PubMed  CAS  Google Scholar 

  60. Hartstein, A I, Morthland, V H., Eng, S., Archer, G. L., Schoenknecht, F D, and Rashad, A L (1989) Restriction enzyme analysis of plasmid DNA and bacteriophage typing of paired Staphylococcus aureus blood culture isolates. J. Clin Microbiol 27, 1874–1879

    PubMed  CAS  Google Scholar 

  61. Coia, J. E., Thomson-Carter, T., Baud, D, and Platt, D. J (1990) Charactertsation of methicillin-resistant Staphylococcus aureus by biotyping, immunoblotting and restriction enzyme fragmentation patterns J Med Microbiol 31, 125–132

    Article  PubMed  CAS  Google Scholar 

  62. Hartstein, A I., Phelps, C L., Kwok R. Y Y, and Mulligan, M. E (1995) In viva stability and discriminatory power of methicillin-resistant Staphylococcus aureus typing by restriction endonuclease analysis of plasmid DNA compared with those of other molecular methods. J Clin. Microbiol 33, 2022–2026

    PubMed  CAS  Google Scholar 

  63. Udo, E E., Pearman, J W., and Grubb, W B. (1993) Genetic analysis of community isolates of methicillin-resistant Staphylococcus aureus in Western Australi J Hosp Infect 25, 97–108

    Article  PubMed  CAS  Google Scholar 

  64. Trilla, A., Nettleman, M. D., Holhs, R. J, Fredrickson, M, Wenzel, R. P, and Pfaller, A (1993) Restriction endonuclease analysis of plasmid DNA from methicillin-resistant Staphylococcus aureus clinical application over a three-year period. Infect Control Hosp Epidemiol 14, 29–35

    Article  PubMed  CAS  Google Scholar 

  65. Sabria-Leal, M, Morthland, V H, Pedro-Boter, M L., Sopena, N, Gimenez-Perez, M, Branchini, M. L M, and Pfaller, M. A (1994) Molecular epidemiology for local outbreaks of methicillin resistant Staphylococcus aureus (MRSA) Eur. J Epidemiol 10, 325–330

    Article  PubMed  CAS  Google Scholar 

  66. Fang, F C, McClelland, M., Gurney, D G, Jackson, M M, Hartstein, A. I, Morthland, V. H., Davis, C E, McPherson, D. C, and Welsh J. (1993) Value of molecular epidemiologic analysis in a nosocomial methicillin-resistant Staphylococcus aureus outbreak. JAMA 270, 1323–1328.

    Article  PubMed  CAS  Google Scholar 

  67. Yuk-Fong, Liu P., Shi, ZY, Lau, YJ, Hu, BS., Shyr, JM., Tsar, WH., Lin, YH, and Tseng, CY. (1996) Use of restriction endonuclease analysis of plasmids and pulsed-field gel electrophoresis to investigate outbreaks of methicillin-resistant Staphylococcus aureus infection Clin Infect Do 22, 86–90

    Article  Google Scholar 

  68. Grattard, F, Etienne, J, Pozzetto, B., Tardy, F, Gaudm, 0 G, and Fleurette, J (1993) Characterization of unrelated strains of Staphylococcus schleiferi by using ribosomal DNA fingerprinting, DNA restriction patterns, and plasmid profiles. J Clin. Microbiol 31, 812–818

    PubMed  CAS  Google Scholar 

  69. Burnie, J P., Matthews, R. C., Lee, W., and Murdoch, D. (1989) A comparison of immunoblot and DNA restriction patterns in characterising methicillin-resistant isolates of Staphylococcus aureus. J Med Microbiol 29, 255–261

    Article  CAS  Google Scholar 

  70. Hall, L M. C, Jordens, J Z., and Wang, F. (1989) Methicillin-resistant Staphylococcus aureus from China characterized by digestion of total DNA with restriction enzymes.Epidemiol Infect 103, 183–192.

    Article  PubMed  CAS  Google Scholar 

  71. Struelens, M. J, Deplano, A, Godard, C, Maes, N., and Serruys, E (1992) Epidemiologic typing and delineation of genetic relatedness of methicillin-resistant Staphylococcus aureus by macrorestriction analysis of genomic DNA by using pulsed-field gel electrophoresis. J Clin Microbiol 30, 2599–2605

    PubMed  CAS  Google Scholar 

  72. Matthews, K R, Jayarao, B M, and Oliver, S P (1992) Restriction endonuclease fingerprinting of genomic DNA of Staphylococcus species of bovine ongin Epidemiol Infect 109, 59–68

    PubMed  CAS  Google Scholar 

  73. Jordens, J Z, and Hall, L M C. (1988) Characterisation of methicillin-resistant Staphylococcus aureus isolates by restriction endonuclease digestion of chromosomal DNA J Med Microbiol 27, 117–123

    Article  PubMed  CAS  Google Scholar 

  74. Renaud, F, Freney, J, Etienne, J, Bes, M., Brun, Y, Barsotti, O, Andre, S., and Fleurette, J (1988) Restriction endonuclease analysis of Staphylococcus epidermidisDNA may be a useful epidemiological marker J Clin Microbiol 26, 1729–1734

    PubMed  CAS  Google Scholar 

  75. Low, D E, Schmidt, B K, Kirpalam, H M, Moodie, R, Kreiswirth, B, Matlow, A, and Ford-Jones, E L (1992) An endemic strain of Staphylococcus haemolyticus colonizing and causing bacteremia in neonatal intensive care unit patients Pediatrics 89, 696–700

    PubMed  CAS  Google Scholar 

  76. Tenover, F C., Arbett, R, Archer, G, Biddle, J, Byrne, S, Goering, R, Hancock, G, etal (1994) Comparison of traditional and molecular methods of typing isolates of Staphylococcus aureus J Clin Microbiol 32, 407–415

    PubMed  CAS  Google Scholar 

  77. Preheim, L, Pitcher, D, Owen, R, and Cookson, B (1991)Typing of methicillin resistant and susceptible Staphylococcus aureus strains by ribosomal RNA gene restriction patterns using a biotinylated prob Eur J EClin Microbiol Infect Dis) 10, 428436

    Google Scholar 

  78. Blumberg, H M, Rimland, D, Kiehlbauch, J A, Terry, P M, and Wachsmuth, I K (1992) Epidemiologic typing of Staphylococcus aureus by DNA restriction fragment length polymorphisms of rRNA genes elucidation of the clonal nature of a group of bacteriophage-nontypeable, ciprofloxacin-resistant, methicillin-susceptible S aureus isolates J. Clin Microbiol 30, 362–369.

    PubMed  CAS  Google Scholar 

  79. Monzon-Moreno, C., Aubert, S, Morvan, S., and El Solh, N. (1991) Usefulness of three probes in typing isolates of methicillin-resistant Staphylococcus aureus (MRSA) J Med Microbiol 35 80–88.

    Article  PubMed  CAS  Google Scholar 

  80. de Lencastre, H, Couto, I, Santos, I., Melo-Cristino, J, Torres-Perena, A, and Tomasz, A (1994) Methicillin-resistant Staphylococcus aureus disease in a Portuguese hospital characterization of clonal types by a combination of DNA typing methods Eur J Clin Infect Dis 13, 64–73

    Article  Google Scholar 

  81. Prevost, G., Jaulhac, B., and Piemont, Y (1992) DNA fingerprinting by pulsed-field gel electrophoresis is more effective than ribotyping in distinguishing among methicillin-resistant Staphylococcus aureus isolates J Clin Microbiol 30, 967–973.

    PubMed  CAS  Google Scholar 

  82. van Belkum, A, Bax, R, and Prevost, G (1994) Comparison of four genotyping assays for epidemiological study of methicillin-resistant Staphylococcus aureus Eur. J Clin Microbiol. Infect, Dis 13, 420–424.

    Article  Google Scholar 

  83. Walcher-Salesse, S, Monzon-Moreno, C., Aubert, S., and El Solh N. (1992) An epidemiological assessment of coagulase-negative staphylococci from an Intensive care unit J Med Microbiol 36, 32l–331

    Google Scholar 

  84. Crichton, P B, Anderson, L. A, Philips, G, Davey, P. G, and Rowley, D I (1995) Subspecies discrimination of staphylococci from revision arthroplasties by ribotyping J Hosp Infect 30, 139–147.

    Article  PubMed  CAS  Google Scholar 

  85. Izard, N C, Hachler, H, Grehn, M, and Kayser, F. H (1992) Ribotyping of coagulase-negative staphylococci with special emphasis on intraspecific typing of Staphylococcus epidermidis J Clin Microbiol 30, 817–823.

    PubMed  CAS  Google Scholar 

  86. Degener, J. E, Heck, M. E. 0. C, vanLeeuwen, W J., Heemskerk, C., Crielaard, A., Joosten, P, and Caesar, P. (1994) Nosocomial infection by Staphylococcus haemolyticus and typing methods for epidemiological study.J. Clin Microbiol 32, 2260–2265.

    PubMed  CAS  Google Scholar 

  87. Smeltzer, M. S., Pratt, F. L., Gillaspy, F G., and Young, L A (1996) Genomic fingerprinting for epidemiological differentiation of Staphylococcus aureus clinical isolates [itJ Clin Microbiol) 34, 1364–1372

    CAS  Google Scholar 

  88. Derbise, A, Dyke, K G. H., and El Solh, N. (1994) Isolation and characterization of IS1181, an insertion sequence from Staphylococcus aureus Plasmid 31, 251–264.

    Article  CAS  Google Scholar 

  89. Teixeira, L A, Resende, C A, Ormonde, L. R., Rosenbaum, R., Figueiredo, A M. S., de Lencastre, H., and Tomasz, A. (1995) Geographic spread of epidemic multireststant Staphylococcus aureus clone in Brazil.J Clin Microbiol 33, 2400–2404

    PubMed  CAS  Google Scholar 

  90. Kreiswirth, B N, Lutwick, M., Chapnick, E K., Gradon, J. D, Lutwick, L I, Sepkowitz, D V, Eisner, W, and Levi, M. H. (1995) Tracing the spread of methicillin-resistant Staphylococcus aureus by southern blot hybridization using gene-specific probes of mec and Tn.554. Microbial Drug Res 1, 307–313.

    Article  CAS  Google Scholar 

  91. Angeles Dommguez, M., de Lencastre, H, Linares, J, and Tomasz, A (1994) Spread and maintenance of a dominant methicillin-resistant Staphylococcus aureus (MRSA) clone during an outbreak of MRSA disease in a Spanish hospital J Clin Microbiol 32, 2081–2087

    Google Scholar 

  92. Wei, M Q, Groth, D. M, Mendis, A H W, Sampson, J, Wetherall, J D, and Grubb, W B. (1992) Typing of methicillin-resistant Staphylococcus aureus with an M13 repeat probe J Hosp Infect 20, 233–245

    Article  PubMed  CAS  Google Scholar 

  93. Wei, M Q, Udo, E E, and Grubb, W B (1992) Typing of methicillin-resistant Staphylococcus aureus with IS256 FEMS Microbiol Lett 99, 175–180

    Article  Google Scholar 

  94. Santos Sanches, I., Ramirez, M, Tram, H., Abecassis, M, Padua, M, Tomasz, A., and de Lencastre, H. (dy1995) Evidence for the geographic spread of a methicillin resistant Staphylococcus aureus clone between Portugal and Spain. J Clin Microbiol. 33, 1243–1246.

    Google Scholar 

  95. Nesin, M., ProJan, S. J., Kreiswirth, B, Bolt, Y., and Novick, R P (1995) Molecular epidemiology of Staphylococcus epidermidis blood isolates from neonatal intensive care unit patients.J. Hosp. Infect 31, 11l–121.

    Article  Google Scholar 

  96. El-Adhami, W, Roberts, L., Vickery, A., Inglis, B., Gibbs, A, and Stewart, P R (1991) Epidemiological analysis of a methicillin-resistant Staphylococcus aureus outbreak using restriction fragment length polymorphisms of genomic DNA.J Gen Microbiol 137, 2713–2720

    PubMed  CAS  Google Scholar 

  97. Ichiyama, S, Ohta, M., Shimokata, K, Kato, N., and Takeuchi, J (1991) Genomic DNA fingerprinting by pulsed-field gel electrophorests as an epidemiological marker for study of nosocomial infections caused by methicillin resistant Staphylococcus aureus J Clin. Microbiol 29.269–2695.

    Google Scholar 

  98. Wet, M Q, Wang, FU, and Grubb, W B (1992) Use of contour-clamped homogeneous electric field (CHEF) electrophorests to type methicillin-resistant Staphylococcus aureus J Med Microbiol 36, 172–176.

    Article  Google Scholar 

  99. Kluytmans, J, van Leeuwen, W, Goessens, W, Hollis, R., Messer, S., Herwaldt, L, Bruming, H, Heck, M, Rost, J, van Leeuwen, N, van Belkum, A, and Verbrugh, H (1995) Food-initiated outbreak of methicillin-resistant Staphylococcus aureus analyzed by pheno-and genotyping.J Clin Microbiol 33, 1121–1128

    PubMed  CAS  Google Scholar 

  100. Trzcinski, K, Hryniewicz, W, Claus, H, and Witte, W (1994) Characterzation of two different clusters of clonally related methicillin-resistant Staphylococcus aureus strams by conventional and molecular typing J Hosp Infect 28, 113–126

    Article  PubMed  CAS  Google Scholar 

  101. Jorgensen, M, Givney, R., Pegler, M, Vickery, A, and Funnell, G (1996) Typing multidrug-resistant Staphylococcus aureus conflicting epidemiological data produced by genotypic and phenotypic methods clarified by phylogenetic analysis J Clin Microbiol 34, 398–403

    PubMed  CAS  Google Scholar 

  102. Cookson, B D., Aparicio, P, Deplano, A, Struelens, M, Goering, R, and Marples, R (1996) Inter-centre comparison of pulsed-field gel electrophorests for the typing of methicillin-resistant Staphylococcus aureus J Med Microbiol 44, 179–184.

    Article  CAS  Google Scholar 

  103. Goering, R V and Duensing, T D (1990) Rapid field inversion gel electrophoresis in combination with an rRNA gene probe in the epidemiological evaluation of staphylococct J Clin Microbiol 28, 426–429

    PubMed  CAS  Google Scholar 

  104. Prevost, G., Pottecher, B, Dahlet, M, Bientz, M, Mantz, J M, and Piemont, Y (1991) Pulsed field gel electrophoresis as a new epidemiological tool for monitoring methicillin-resistant Staphylococcus aureus in an intensive care unit J Hosp Infect 17, 255–269

    Article  PubMed  CAS  Google Scholar 

  105. Schlichting, C, Branger, C, Fournier, J. M., Witte, W, Boutonnier, A, Wolz, C., Goullet, P, and Doring, G (1993) Typing of Staphylococcus aureus by pulsed-field gel electrophoresis, zymotyping, capsular typing, and phage typing: resolution of clonal relationships.J Clin Mizrobiol 31, 227–232

    CAS  Google Scholar 

  106. Nada, T., Ichiyama, S., Osada, Y., Ohta, M., Shimokata, K, Kato, N, and Nakashima, N (1996) Comparison of DNA fingerprinting by PFGE and PCR-RFLP of the coagulase gene to distinguish MRSA isolates J Hosp Infect 32, 305–317

    Article  PubMed  CAS  Google Scholar 

  107. Couto, I, Melo-Cristino, J, Fernandes, M L, Garcia, T, Serrano, N., Saldago, M J, Torres-Pereira, A., Santos Sanches, I, and de Lencastre, H (1995) Unusually large number of methicillin-resistant Staphylococcus aureus clones in a Portuguese hospital.J Clin Microbiol 33, 2032–2035

    PubMed  CAS  Google Scholar 

  108. Carles-Nurit, M. J., Christophle, B, Broche, S., Gouby, A, Bouziges, N, and Ramuz, M (1992) DNA polymorphisms in methicillin-susceptible and methicillin-resistant strains of Staphylococcus aureus. J Clin Microbiol 30, 2092–2096.

    PubMed  CAS  Google Scholar 

  109. Pantucek, R., Gotz, F, Doskar, J, and Rosypal, S (1996) Genomic variability of Staphylococcus aureus and the other coagulase-positive Staphylococcus specues estimated by macrorestriction analysis using pulsed-field gel electrophoresis Int J Syst Bacteriol 46, 216–222

    Article  PubMed  CAS  Google Scholar 

  110. Huebner, J, Pier, G B, Maslow, J. N., Muller, E., Shiro, H., Parent, M., Kropec, A., Arbeit, R. D., and Goldmann, D. A. (1994) Endemic nosocomial transmission of Staphylococcus epidermidis bacteremia isolates in a neonatal intensive care unit over 10 years. J Infect Dis 169, 526–531

    Article  PubMed  CAS  Google Scholar 

  111. Witte, W., Cuny, Ch., and Claus, H. (1993) Clonal relatedness of Staphylococcus aureus strains from Infections in humans as deduced from genomic DNA fragment patterns.Med Microbiol Lett 2, 72–79

    Google Scholar 

  112. Goering, R V. and Winters, M A (I992) Rapid method for epidemiological evaluation of Gram-positive cocci by field inversion gel electrophoresis J Clin Microbiol. 30, 577–580

    PubMed  CAS  Google Scholar 

  113. Goh, S. H., Byrne, S K, Zhang, J. L, and Chow, A W (1992) Molecular typing of Staphylococcus aureus on the basis of coagulase gene polymorphisms.J Clin Microbiol.30, 1642–1645

    PubMed  CAS  Google Scholar 

  114. Schwarzkopf, A., Karch, H., Schmidt, H., Lenz, W., and Heesemann, J (1993) Phenotypical and genotypical characterization of epidemic clumping factor-negative, oxacillin-resistant Staphylococcus aureus. J. Clin. Microbiol.31, 2281–2285.

    PubMed  CAS  Google Scholar 

  115. Schwarzkopf, A and Karch, H (1994) Genetic variation in Staphylococcus aureus coagulase genes potential and limits for use as epidemiological marker J Clin Microbiol 32, 2401–2412.

    Google Scholar 

  116. Frenay, H. M E, Theelen, J. P G., Schouls, L. M., Vandenbroucke-Grauls, M J. E., Verhoef, J., Van Leeuwen, W. J., and Mooi, F. R. (1994) Discrimination of epidemic and nonepidemic methicillin-resistant Staphylococcus aureus strains on the basis of protein A gene polymorphism J. Clin Microbiol 32, 846, 847

    PubMed  CAS  Google Scholar 

  117. Frenay, H. M. E, Bunschoten, A. E., Schouls, L M, van Leeuwen, W J, Vandenbroucke-Grauls, C M. J E., Verhoef, J., and Mool, F R (1996) Molecular typing of methicillin-resistant Staphylococcus aureus on the basis of protein A gene polymorphism.Eur J Clin. Microbiol Infect DIS 15, 60–64

    Article  PubMed  CAS  Google Scholar 

  118. Nishi, JI, Miyanohara, H, Nakajima, T, Kitajima, I., Yoshinaga, M, Maruyama, I, and Miyata, K. (1995) Molecular typing of the methicllin resistance determinant (mec) of clinical strains of Staphylococcus based on mec hypervariable region length polymorphisms.J. Lab. Clin. Med 126, 29–35.

    PubMed  CAS  Google Scholar 

  119. DelVecchio, V. G, Petroziello, J. M., Gress, M J., McCleskey, F. K, Melcher, G. P., Crouch, H, K, and Lupski, J. R. (1995) Molecular genotyping of methiclllin resistant Staphylococcus aureus via fluorophore-enhanced repetitive sequence PCR. J. Clin Microbiol 33, 2141–2144.

    CAS  Google Scholar 

  120. Gurtler, V. and Bane, H. D. (1995) Typing of Staphylococcus aureus strains by PCR-amplification of variable-length 16S–23S rDNA spacer regions characterization of spacer sequences.Microbiology 141, 1255–1265

    Article  PubMed  CAS  Google Scholar 

  121. Cuny, C. and Witte, W. (1996) Typing of Staphylococcus aureus by PCR for DNA sequences flanked by transposon Tn916 target region and ribosomal binding site. J Clin. Mirobiol 34, 1502–1505.

    CAS  Google Scholar 

  122. Deplano, A., Vanechoute, M., Verschrogen, G., and Struelens, N. J. (1997) Typing of Staphylococcus aureus and Staphylococcus epidermis strains by PCR analysis of inter-IS256 sporer length polymorphism J. Clin. Microbiol 35, 2580–2587

    PubMed  CAS  Google Scholar 

  123. van Belkum, A., Bax, R., Peerbooms, P, Goessens, W. H F, vanLeeuwen, N., and Quint, W G V (1993) Comparison of phage typing and DNA fingerprinting by polymerase chain reaction for discrimination of methicillin-resistantstaphylococcus aureus strains. J Clin. Microbiol 31, 798–803

    PubMed  Google Scholar 

  124. Saulnier, P., Bourneix, C., Prevost, G., and Andremont, A (1993) Random amplified polymorphic DNA assay is less discriminant than pulsed-field gel electrophoresis foi typing strains of methicillin-resistant Staphylococcus aureus J Clin Microbiol 31, 982–985

    PubMed  CAS  Google Scholar 

  125. Struelens, M. J, Bax, R, Deplano, A., Quint, W G V., and vanBelkum, A (1993)Concordant clonal delineation of methicillin-resistantStaphylococcus aureus chain reaction genomic fingerprinting. J Clin. Microbiol 31, 1964–1970

    PubMed  CAS  Google Scholar 

  126. van Belkum, A, Kluytmans, J., van Leeuwen, W, Bax, R, Quint, W, Peters, E, Fluit, A D., Vandenbroucke-Grauls, C., van den Brule, A., Koeleman, H, Melchers, W, Meis, J, Elaichoum, A, Vaneechoutte, M., Moonens, F, Maes, N., Struelens, M, Tenover, F., and Verbrugh, H (1995) Multicenter evaluation of arbitrarily primed PCR for typing of Staphylococcus aureus strains J Clin Microbiol 33, 1537–1547

    PubMed  Google Scholar 

  127. Bingen, E, Barc, MC, Brahimi, N, Vilmer, E., and Beaufils, F (1995) Randomly amplified polymorphic DNA analysis provides rapid differentiation of methicillin-resistant coagulase-negativestaphylococcus bacteremia isolates in a pediatric hospital J Clin Microbiol 33, 1657–1659

    PubMed  CAS  Google Scholar 

  128. Jordens, J. Z. and Hall, L M C. (1989) Chromosomally-encoded gentamicin resistance in “epidemic” methicillin-resistant Staphylococcus aureus’ detection with a synthetic oligonucleotide probe. J. Antimicrob Chemother 23, 327–334

    Article  PubMed  CAS  Google Scholar 

  129. Vanhoof, R., Godard, C, Content, J, Nyssen, H J, Hannecart-Pokorni, E., and theBelgian Study Group of Hospital Infections (GDEPIH/GOSPIZ) (1994) Detection by polymerase chain reaction of genes encoding aminoglycoside-modifying enzymes in methicillin-resistant Staphylococcus aureus isolates of epidemic phage types.J Med Microbiol.41, 282–

    Article  PubMed  CAS  Google Scholar 

  130. Arthur, M., Molinas, C, and Mabilat, C. (1993) PCR detection of erm erythromycin resistance genes by using degenerate oligonucleotide primers. in Diagnostic Molecular Microbiology Principles and Applications, (Persing, D H., ed), American Society for Microbiology, Washington, DC.

    Google Scholar 

  131. Ferrero, L., Cameron, B, and Crouzet, J (1995) Analysis of gyrA mutations in stepwise-selected ciprofloxacin-resistant mutants of Staphylococcus aureus Antimicrob. Agents Chemother 39, 1554–1558

    CAS  Google Scholar 

  132. Tokue, Y., Sugano, K., Saito, D., Noda, T., Ohkura, H., Shimosato, Y., and Sekiya, T. (1994) Detection of novel mutations in the gyr A gene of Staphylococcus aureus by nonradioisotopic single-strand conformation polymorphism analysis and direct DNA sequencmg. Antimicrob Agents Chemother 38, 428–431

    PubMed  CAS  Google Scholar 

  133. Goswitz, J. J., Willard, K E., Fasching, C. E, and Peterson, L. R (1992) Detection of gyr A gene mutations associated with ciprofloxacin resistance in methicillin-resistant Staphylococcus aureus: analysis by polymerase chain reaction and automated direct DNA sequencing Antimicrob Agents Chemother 36, 1166–1169

    PubMed  CAS  Google Scholar 

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  1. Unite di Epidemiologie et diHygiene Hospitaliere, Laboratoire de Microbiologie, Hospital Erasma, Bruxelles, Belgium

    Ariane Deplano & Marc J. Struelens

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  1. Ariane Deplano
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  2. Marc J. Struelens
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  1. Central Public Health Laboratory, London, UK

    Neil Woodford  & Alan P. Johnson  & 

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Deplano, A., Struelens, M.J. (1998). Nosocomial Infections Caused by Staphylococci. In: Woodford, N., Johnson, A.P. (eds) Molecular Bacteriology. Methods in Molecular Medicine™, vol 15. Humana Press. https://doi.org/10.1385/0-89603-498-4:431

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  • DOI: https://doi.org/10.1385/0-89603-498-4:431

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-498-3

  • Online ISBN: 978-1-59259-599-0

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