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Measurement of Recovery of Function Following Whole Muscle Transfer, Myoblast Transfer, and Gene Therapy

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Tissue Engineering Methods and Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 18))

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Abstract

For a skeletal muscle tissue engineer, the most important issue following an experimental intervention is the evaluation of the recovery of the functional capabilities of the tissue, relative to those of the control tissue. Whether investigators perform whole muscle transfers with spontaneous (1) or surgical (2) vascular and nerve repair, myoblast transfers (3), or manipulations of muscle-specific genes (4,5), the question remains the same: Has the intervention impaired, maintained, or enhanced the functional capabilities of the skeletal muscles involved? In each case, determining structure–function relationships is of vital importance because structure–function relationships are frequently disrupted following an intervention, so that muscle mass and total muscle fiber cross-sectional area (CSA) are not different from control values, but function is impaired, or both are impaired, but with different magnitudes of impairment.

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Author information

Authors and Affiliations

  1. Institute of Gerontology, University of Michigan, Ann Arbor, MI

    John A. Faulkner, Susan V. Brooks & Robert G. Dennis

Authors
  1. John A. Faulkner
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  2. Susan V. Brooks
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  3. Robert G. Dennis
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Editor information

Editors and Affiliations

  1. Center for Engineering in Medicine, Massachusetts General Hospital/Harvard Medical School, Boston, MA

    Jeffrey R. Morgan PhD  & Martin L. Yarmush MD  & 

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© 1999 Humana Press Inc., Totowa, NJ

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Faulkner, J.A., Brooks, S.V., Dennis, R.G. (1999). Measurement of Recovery of Function Following Whole Muscle Transfer, Myoblast Transfer, and Gene Therapy. In: Morgan, J.R., Yarmush, M.L. (eds) Tissue Engineering Methods and Protocols. Methods in Molecular Medicine™, vol 18. Humana Press. https://doi.org/10.1385/0-89603-516-6:155

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  • DOI: https://doi.org/10.1385/0-89603-516-6:155

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-516-4

  • Online ISBN: 978-1-59259-602-7

  • eBook Packages: Springer Protocols

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