Abstract
Monitoring microbial activities in the environment is difficult owing to the lack of suitable methods. For a technique to be useful for monitoring in situ activities, it must possess the following properties: sensitivity, selectivity, stability, the ability to make continuous real-time measurements, and be noninvasive or perturbing to the microorganisms or to the environment being studied. Currently used methods include manometric techniques, microsensors, chemical assays, gas chromatography and high-performance liquid chromatography, but all have their limitations and usually require substantial disruption to the environment being studied. The principles of membrane inlet mass spectrometry (MIMS) have been described elsewhere (1,2), and are summarized in detail here. Although MIMS allows measurements of numerous gases to be made in both the liquid and gas phases, only liquid phase measurements are detailed in this example.
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References
Lloyd, D. and Scott, R. I. (1983) Direct measurement of dissolved gases in microbial systems using inlet mass spectrometry. J. Gen. Microbiol. Methods 127, 313–328.
Degn, H. (1992) Membrane inlet mass spectrometry in pure and applied microbiology. J. Microbiol. Methods 15, 185–197.
Davies, K. J. P., Lloyd, D., and Boddy, L. (1989) Effect of oxygen on denitrification in Paracoccus denitrificans and Pseudomonas aeruginosa. J. Gen. Microbiol. 135, 2445-2451.
Van Niel, E. W. J., Robertson, L. A., Cox, R. P., and Kuenen, J. G. (1992) Inhibition of denitrification and oxygen utilization by Thiosphaera pantotropha. J. Gen. Appl. Microbiol. 38, 553–558.
Lloyd, D., Boddy, L., and Davies, K. J. P. (1987) Persistence of bacterial denitrification capacity under aerobic conditions: the rule rather than the exception. FEMS Microbiol. Ecol. 45, 185–190.
Lloyd, D. (1993) Aerobic denitrification in soils and sediments: from fallacies to facts. Trends Ecology Evol. 8, 352–356.
Thomas, K. L., Lloyd, D., and Boddy, L. (1994) Effects of oxygen, pH and nitrate concentration on denitrification by Pseudomonas species. FEMS Microbiol. Lett. 118, 181–186.
Thomsen, J. K., Geest, T., and Cox, R. P. (1994) Mass spectrometric studies of the effect of pH on the accumulation of intermediates in denitrification by Paracoccus denitrificans. Appl. Environ. Microbiol. 60, 536–541.
Thomas, K. L. and Lloyd, D. (1995) Measurement of denitrification in estuarine sediment using membrane inlet mass spectrometry. FEMS Microbiol. Ecol. 16, 103–114.
Degn, H., Cox, R. P., and Lloyd, D. (1985) Continuous measurement of dissolved gases in biological systems with the quadrupole mass spectrometer. Methods Biochem. Anal. 31, 185–197.
Jouanneau, Y., Kelley, B. C., Berlier, Y., Lespinat, P. A., and Vignais, P. M. (1980) Continuous monitoring by mass spectrometry of H2 production and recycling in Rhodopseudomonas capsulate. J. Bacteriol. 143, 628–636.
Berlier, Y. M. and Lespinat, P. A. (1980) Mass spectrometric kinetic studies of the nitrogenase and hydrogenase activities in in-vivo cultures of Azospirillum brasilense Sp.7. Arch. Microbiol. 125, 67–72.
Jensen, B. B., Cox, R. P., and Degn, H. (1981) Mass spectrometric measurements of steady-state kinetics of cyanobacterial nitrogen fixation by monitoring dissolved N2 in an open system. FEMS Microbiol. Lett. 12, 37–40.
Jensen, B. B. and Cox, R. P. (1988) Measurement of hydrogen exchange and nitrogen uptake by mass spectrometry. Methods Enzymol. 167, 467–474.
Carlsen, H. N., Degn, H., and Lloyd, D. (1991) Effects of alcohols on the respiration and fermentation of aerated suspensions of Baker’s Yeast. J. Gen. Microbiol. 137, 2879–2883.
Bohátka,. S., Futó, I., Gál, I., Gál, J., Langer, G., Molnár, J., Paál, A., Pintér, G., Simon, M., Szádai, J., Székely, G., and Szilágyi, J. (1993) Quadrupole mass spectrometer system for fermentation monitoring. Vacuum 44, 669–671.
Lloyd, D., Ellis, J. E., Hillman, K., and Williams, A. G. (1992) Membrane inlet mass spectrometry: probing the rumen ecosystem. Journal of Applied Bacteriology Symposium Supplement 1992 73, 155S–163S.
Virkki, V. T., Ketola, R. A., Ojala, M., Kotiaho, T., Komppa, V., Grove, A., and Facchetti, S. (1995) On-site environmental analysis by membrane inlet mass spectrometry. Analytical Chemistry 67, 1421–1425.
Harland, B. J. and Nicholson, P. J. (1993) Continuous measurement of volatile organic chemicals in natural waters. Sci. Total Environ. 135, 37–54.
Cristea, O. and Langer, G. (1992) Gas metabolism measurements of aquatic living structures by membrane inlet mass spectrometry for water pollution detection. Sensors and Actuators B 7, 518–521.
Benstead, J. and Lloyd, D. (1994) Direct mass spectrometric measurement of gases in peat cores. FEMS Microbiol. Ecol. 13, 233–240.
Lauritsen, F. R. and Gylling, S. (1995) On-line monitoring of biological reactions at low parts-per-trillion levels by membrane inlet mass spectrometry. Analytical Chemistry 67:1418–1420.
Lloyd, D., Davies, K. J. P., and Boddy, L. (1986) Mass spectrometry as an ecological tool for in situ measurement of dissolved gases in sediment systems. FEMS Microbiol. Ecol. 38, 11–17.
Wilhelm, E., Battino, R., and Wilcock, R. J. (1977) Low pressure solubility of gases in liquid water. Chem. Rev. 77, 219–230.
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© 1999 Humana Press Inc., Totowa, NJ
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Firth, J.R., Edwards, C. (1999). Monitoring Microbial Activities Using Membrane Inlet Mass Spectrometry. In: Edwards, C. (eds) Environmental Monitoring of Bacteria. Methods in Biotechnology, vol 12. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-566-2:267
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DOI: https://doi.org/10.1385/0-89603-566-2:267
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-566-9
Online ISBN: 978-1-59259-487-0
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