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Generation of HLA Class I Transfected Target Cell Lines

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Natural Killer Cell Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 121))

Abstract

Normal human lymphoblastoid cell lines (LCLs) express six classical class I HLA genes from three gene loci, HLA-A, HLA-B, and HLA-C, which are located within the major histocompatibility complex (MHC). Because of the extensive allelic polymorphism of HLA class I genes, cell lines from heterozygous individuals can express up to six different class I alleles (1). Class I phenotypes, as well as other properties of different lymphoblastoid target cells, can therefore be very heterogeneous. Hence, in investigating the effects on and specificity of class I recognition by natural killer (NK) cells, it has proved useful to use transfected target cells that express only one class I allele, so that the effects of individual class I allotypes can be compared directly using the same target cell background.

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Author information

Authors and Affiliations

  1. Division of Rheumatology, Immunology, and Allergy, Department of Medicine, Brigham and Women′s Hospital and Harvard Medical School, Boston, MA

    Jenny E. Gumperz

Authors
  1. Jenny E. Gumperz
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Editor information

Editors and Affiliations

  1. Fox Chase Cancer Center, Philadelphia, PA

    Kerry S. Campbell

  2. Basel Institute for Immunology, Basel, Switzerland

    Marco Colonna

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© 1999 Humana Press Inc.

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Gumperz, J.E. (1999). Generation of HLA Class I Transfected Target Cell Lines. In: Campbell, K.S., Colonna, M. (eds) Natural Killer Cell Protocols. Methods in Molecular Biology, vol 121. Humana Press. https://doi.org/10.1385/1-59259-044-6:49

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  • DOI: https://doi.org/10.1385/1-59259-044-6:49

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-683-3

  • Online ISBN: 978-1-59259-044-5

  • eBook Packages: Springer Protocols

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