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Assay of Malate Dehydrogenase

A Substrate for the E. coli Chaperonins GroEL and GroES

  • Protocol
Chaperonin Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 140))

Abstract

Malate dehydrogenase (MDH) is found in all eukaryotic cells as two isozymes:mitochondrial (mMDH) and cytoplasmic (soluble, sMDH) (1). In constrast, prokaryotes contain only a single form of MDH. The crystal structures of MDH from Escherichia coli (2 3), porcine cytoplasm (4), porcine mitochondria (5 6), and Thermus flavus (7) have been solved and are essentially identical. Refer to ref. (8) for a comprehensive review on MDH.

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Authors and Affiliations

  1. Department of Cellular Biochemistry, Max Planck Institute of Biochemisty, Martinsried, Germany

    Manajit Hayer-Hartl

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  1. Manajit Hayer-Hartl
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Editors and Affiliations

  1. Max-Planck Institute for Biochemistry, Martinsried, Germany

    Christine Schneider

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© 2000 Humana Press Inc.

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Hayer-Hartl, M. (2000). Assay of Malate Dehydrogenase. In: Schneider, C. (eds) Chaperonin Protocols. Methods in Molecular Biology, vol 140. Humana, Totowa, NJ. https://doi.org/10.1385/1-59259-061-6:127

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  • DOI: https://doi.org/10.1385/1-59259-061-6:127

  • Publisher Name: Humana, Totowa, NJ

  • Print ISBN: 978-0-89603-739-7

  • Online ISBN: 978-1-59259-061-2

  • eBook Packages: Springer Protocols

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