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Neuroepithelial Differentiation Induced by ECM Molecules

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Extracellular Matrix Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 139))

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Abstract

Many important processes in the development of the vertebrate central nervous system (CNS) rely on signals induced by extracellular matrix (ECM) components (1,2). These molecules are known to contain multiple functional domains that deliver distinct signals to the cells with which they interact. We will focus here on a well-known ECM glycoprotein, laminin-1, which plays a variety of roles in the development of the CNS (2).

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References

  1. Adams, J. C. and Watt, F. M. (1993) Regulation of development and differentiation by the extracellular matrix. Development 117, 1183–1198.

    PubMed  CAS  Google Scholar 

  2. Reichardt, L. F. and Tomaselli, K. J. (1991) Extracellular matrix molecules and their receptors: functions in neural development. Annu. Rev. Neurosci. 14, 531–570.

    Article  PubMed  CAS  Google Scholar 

  3. Timpl, R., Rohde, H., Robey, P. G., Rennard, S. I., Foidart, J. M., and Martin, G. R. (1979) Laminin-a glycoprotein from basement membranes. J. Biol. Chem. 254, 9933–9937.

    PubMed  CAS  Google Scholar 

  4. Tryggvason, K. (1993) The laminin family. Curr. Op. Cell Biol. 5, 877–882.

    Article  PubMed  CAS  Google Scholar 

  5. Ehrig, K., Leivo, I., Argraves, W. S., Ruoslahti, E., and Engvall, E. (1990) Merosin, a tissue specific basement membrane protein, is a laminin-like protein. Proc. Natl. Acad. Sci. USA 87, 3264–3268.

    Article  PubMed  CAS  Google Scholar 

  6. Davis, G. E., Manthorpe, M., Engvall, E., and Varon, S. (1985) Isolation and characterization of rat schwannoma neurite-promoting factor: evidence that the factor contains laminin. J. Neurosci. 5, 2662–2671.

    PubMed  CAS  Google Scholar 

  7. Prada, C., Puga, J., Pérez-Méndez, L., López, R., and RamÍrez, G. (1991) Spatial and temporal patterns of neurogenesis in the chick retina. Eur. J. Neurosci. 3, 559–569.

    Article  PubMed  Google Scholar 

  8. Frade, J. M., MartÍnez-Morales, J. R., and RodrÍguez-Tébar, A. (1996a) Laminin-1 selectively stimulates neuron generation from cultured retinal neuroepithelial cells. Exp. Cell Res. 222, 140–149.

    Article  PubMed  CAS  Google Scholar 

  9. Frade, J. M., MartÍ, E., Bovolenta, P., RodrÍguez-Peña, M. A., Pérez-GarcÍa, D., Rohrer, H., et al. (1996b) Insulin-like growth factor-I stimulates neurogenesis in chick retina by regulating expression of the a6 integrin subunit. Development 122, 2497–2506.

    PubMed  CAS  Google Scholar 

  10. Beck, K., Hunter, I., and Engel, J. (1990) Structure and function of laminin: anatomy of a multidomain glycoprotein. FASEB J. 4, 148–160.

    PubMed  CAS  Google Scholar 

  11. Hamburger, V., and Hamilton, H. (1951) A series of normal stages in the development of chick embryo. J. Morphol. 88, 49–92.

    Article  Google Scholar 

  12. Rathjen, F. G., Wolf, J. M., Franck, R., Bonhoeffer, F., and Rutishauser, U. (1987) Membrane glycoproteins involved in neurite fasciculation. J. Cell Biol. 104, 343–353.

    Article  PubMed  CAS  Google Scholar 

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© 2000 Humana Press Inc.

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Frade, J.M., Tébar, A.R. (2000). Neuroepithelial Differentiation Induced by ECM Molecules. In: Streuli, C.H., Grant, M.E. (eds) Extracellular Matrix Protocols. Methods in Molecular Biology™, vol 139. Humana Press. https://doi.org/10.1385/1-59259-063-2:257

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  • DOI: https://doi.org/10.1385/1-59259-063-2:257

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-624-6

  • Online ISBN: 978-1-59259-063-6

  • eBook Packages: Springer Protocols

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