Abstract
It is often desirable to localize a specific protein within a layer, cell, or subcellular organelle in the retina. A variety of immunocytochemical techniques are available to achieve this goal. These techniques all utilize an antibody that binds to the protein of interest and is detected by a label (a fluorescent dye, enzyme, or electron dense marker) to localize the specific antigen in the tissue.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Matsumoto, B. and Hale, I. L. (1993) Resolution of subcellular detail in thick tissue sections: immunohistochemical preparation and fluorescence confocal microscopy, in Cell Biological Applications of Confocal Microscopy (Matsumoto, B., ed.), Academic, San Diego, CA, pp. 289–324.
Matsumoto, B. and Hale, I. L. (1993) Preparation of retinas for studying photoreceptors with confocal microscopy, in Methods in Neurosciences, vol. 15 (Hargrave, P. A., ed.), Academic, San Diego, CA, pp. 54–71.
Milam, A. H., Saari, J. C., Jacobson, S. G., et al. (1993) Autoantibod-ies against retinal bipolar cells in cutaneous melanoma-associated retinopathy. Invest. Ophthalmol. Vis. Sci. 149, 574–576.
Bullock, G. R. and Petrusz, P. (1989) Techniques in Immuno-cytochemistry. Academic, San Diego, CA.
Bunt-Milam, A. H., Saari, J. C., Klock, I. B., and Garwin, G. G. (1985) Zonulae adherentes pore size in the external limiting membrane of the rabbit retina. Invest. Ophthalmol. Vis. Sci. 26, 1377–1380.
Verkleij, A. J. and Leunissen, J. L. M. (1989) Immuno-Gold Labeling in Cell Biology. CRC, Boca Raton, FL.
Polak, J. M. and Priestley, J. V. (1992) Electron Microscopic Immuno-cy to chemistry, Oxford University Press, New York, NY.
Eldred, W. D., Zucker, C., Karten, H. J., and Yazulla, S. (1983) Comparison of fixation and penetration enhancement techniques for use in ultrastructural immunocytochemistry. J. Histochem. Cytochem. 31, 285–292.
Milam, A. H. and Jacobson, S. G. (1990) Photoreceptor rosettes with blue cone opsin immunoreactivity in retinitis pigmentosa. Ophthalmology 97, 1620–1631.
Milam, A. H., Possin, D. E., Huang, J., et al. (1997) Characterization of aldehyde dehydrogenase-positive amacrine cells restricted in distribution to the dorsal retina. Vis. Neurosci. 14, 601–608.
Shi, S.-R., Imam, A., Young, L., et al. (1995) Antigen retrieval immunohistochemistry under the influence of pH using monoclonal antibodies. J. Histochem. Cytochem. 43, 193–201.
Fariss, R. N., Apte, S. S., Luthert, P. J., et al. (1998) Abnormal accumulation of tissue inhibitor of metalloproteinases-3 in human eyes with Sorsby′s fundus dystrophy and retinitis pigmentosa. Brit. J. Ophthalmol. 82, 1329–1334.
Milam, A. H., Li, Z.-Y., Cideciyan, A. V., and Jacobson, S. G. (1996) Clinicopathologic effects of the Q64ter rhodopsin mutation in retinitis pigmentosa. Invest. Ophthalmol. Vis. Sci. 37, 753–765.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Humana Press Inc.
About this protocol
Cite this protocol
Milam, A.H. (2001). Immunocytochemical Studies of the Retina. In: Rakoczy, P.E. (eds) Vision Research Protocols. Methods in Molecular Medicine™, vol 47. Humana Press. https://doi.org/10.1385/1-59259-085-3:71
Download citation
DOI: https://doi.org/10.1385/1-59259-085-3:71
Publisher Name: Humana Press
Print ISBN: 978-0-89603-837-0
Online ISBN: 978-1-59259-085-8
eBook Packages: Springer Protocols